Invitrogen

Phospho-S6 (Ser244, Ser247) Polyclonal Antibody

3 Published Figures

22 References

View all (72) S6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

Phospho-S6 (Ser244, Ser247) Antibody in Western Blot (WB)

FIGURE: 1 / 4

Phospho-S6 (Ser244, Ser247) Antibody (44-923G) in WB

Peptide Competition. Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 (pSpS244/247) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incub... View More

Phospho-S6 (Ser244, Ser247) Antibody in Immunohistochemistry (IHC)

Phospho-S6 (Ser244, Ser247) Antibody in Immunoprecipitation (IP)

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Product Details

44-923G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 5 publications 5 publications

In Situ Hybridization (ISH) (ISH)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Fish, Human, Mouse, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human RPS6 that contains serines 244 and 247. The sequence is conserved in mouse and rat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533798

Target Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sensing and avoiding sick conspecifics requires Gαi2⁺ vomeronasal neurons.

BMC biology

Weiss J,Vacher H,Trouillet AC,Leinders-Zufall T,Zufall F,Chamero P

44-923G was used in Immunocytochemistry to investigate the role of the Gαi2 vomeronasal subsystem and associated neural circuits in mediating the detection and avoidance of sick conspecifics in mice experiencing acute inflammation induced by lipopolysaccharide (LPS) administration, shedding light on the molecular and neural mechanisms underlying this behavior.

Mon Jul 10 00:00:00 UTC 2023

Rotavirus Downregulates Tyrosine Hydroxylase in the Noradrenergic Sympathetic Nervous System in Ileum, Early in Infection and Simultaneously with Increased Intestinal Transit and Altered Brain Activities.

mBio

Hellysaz A,Svensson L,Hagbom M

44-923G was used in Immunohistochemistry to reveal that the rotavirus-infected gut communicates with the CNS before the onset of diarrhea, a surprising observation that brings a new understanding of how rotavirus gives rise to sickness symptoms.

Wed Oct 26 00:00:00 UTC 2022

Top-down control of conditioned overconsumption is mediated by insular cortex Nos1 neurons.

Cell metabolism

Stern SA,Azevedo EP,Pomeranz LE,Doerig KR,Ivan VJ,Friedman JM

44-923G was used in Immunohistochemistry to indicate that the insular cortex provides top-down control of homeostatic circuits to promote overconsumption in response to learned cues.

Tue Jul 06 00:00:00 UTC 2021

Restriction of food intake by PPP1R17-expressing neurons in the DMH.

Proceedings of the National Academy of Sciences of the United States of America

Caglar C,Friedman J

44-923G was used in Immunohistochemistry to identify potentially novel sites of leptin action by molecularly profiling leptin-responsive neurons in the hypothalamus and brainstem.

Tue Mar 30 00:00:00 UTC 2021

Restriction of food intake by PPP1R17-expressing neurons in the DMH.

Proceedings of the National Academy of Sciences of the United States of America

Caglar C,Friedman J

44-923G was used in Immunohistochemistry to identify potentially novel sites of leptin action by molecularly profiling leptin-responsive neurons in the hypothalamus and brainstem.

Tue Mar 30 00:00:00 UTC 2021

Hippocampal neurons' cytosolic and membrane-bound ribosomal transcript profiles are differentially regulated by learning and subsequent sleep.

Proceedings of the National Academy of Sciences of the United States of America

Delorme J,Wang L,Kodoth V,Wang Y,Ma J,Jiang S,Aton SJ

44-923G was used in Immunoprecipitation to study how transcript profiles change as a function of sleep versus sleep deprivation and prior learning (contextual fear conditioning).

Tue Nov 30 00:00:00 UTC 2021

Urocortin-3 neurons in the mouse perifornical area promote infant-directed neglect and aggression.

eLife

Autry AE,Wu Z,Kapoor V,Kohl J,Bambah-Mukku D,Rubinstein ND,Marin-Rodriguez B,Carta I,Sedwick V,Tang M,Dulac C

44-923G was used in Immunoprecipitation to show infant-directed attacks correlate with urocortin-3 expression in neurons in the hypothalamic perifornical area.

Mon Aug 23 00:00:00 UTC 2021

PNOC^ARC Neurons Promote Hyperphagia and Obesity upon High-Fat-Diet Feeding.

Neuron

Jais A,Paeger L,Sotelo-Hitschfeld T,Bremser S,Prinzensteiner M,Klemm P,Mykytiuk V,Widdershooven PJM,Vesting AJ,Grzelka K,Minère M,Cremer AL,Xu J,Korotkova T,Lowell BB,Zeilhofer HU,Backes H,Fenselau H,Wunderlich FT,Kloppenburg P,Brüning JC

44-923G was used in Immunoprecipitation to characterize PNOCARC neurons as a novel ARC neuron population activated upon palatable food consumption to promote hyperphagia.

Wed Jun 17 00:00:00 UTC 2020

MCH Neurons Regulate Permeability of the Median Eminence Barrier.

Neuron

Jiang H,Gallet S,Klemm P,Scholl P,Folz-Donahue K,Altmüller J,Alber J,Heilinger C,Kukat C,Loyens A,Müller-Fielitz H,Sundaram S,Schwaninger M,Prevot V,Brüning JC

44-923G was used in PhosphoRiboTrap Preparation to reveal that MCH neurons directly regulate permeability of the ME barrier, linking the activity of energy state and sleep regulatory neurons to the regulation of hormone accessibility to the ARC.

Wed Jul 22 00:00:00 UTC 2020

Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Håglin S,Berghard A,Bohm S

44-923G was used in Immunohistochemistry-immunofluorescence to support the concept that inositol-1,4,5-trisphosphate type 3 receptor signaling in HBCs, together with altered retinoic acid metabolism within the niche, promote HBC lineage commitment toward two types of respiratory cells that will maintain epithelial barrier function once the capacity to regenerate OE cells ceases.

Wed May 20 00:00:00 UTC 2020

Neural correlates of winning and losing fights in poison frog tadpoles.

Physiology & behavior

Fischer EK,Alvarez H,Lagerstrom KM,McKinney JE,Petrillo R,Ellis G,O'Connell LA

44-923G was used in Immunohistochemistry to suggest the neural correlates of aggression in poison frog tadpoles are similar to neural mechanisms mediating aggression in adults and juveniles of other vertebrate taxa.

Tue Sep 01 00:00:00 UTC 2020

Peripheral oxytocin injection modulates vomeronasal sensory activity and reduces pup-directed aggression in male mice.

Scientific reports

Nakahara TS,Camargo AP,Magalhães PHM,Souza MAA,Ribeiro PG,Martins-Netto PH,Carvalho VMA,José J,Papes F

44-923G was used in Immunohistochemistry to investigate hormonal control of pup-induced activity in the vomeronasal organ, an olfactory sensory structure involved in the detection of non-volatile chemosignals.

Tue Nov 17 00:00:00 UTC 2020

Peripheral oxytocin injection modulates vomeronasal sensory activity and reduces pup-directed aggression in male mice.

Scientific reports

Nakahara TS,Camargo AP,Magalhães PHM,Souza MAA,Ribeiro PG,Martins-Netto PH,Carvalho VMA,José J,Papes F

Tue Nov 17 00:00:00 UTC 2020

A limbic circuit selectively links active escape to food suppression.

eLife

Azevedo EP,Tan B,Pomeranz LE,Ivan V,Fetcho R,Schneeberger M,Doerig KR,Liston C,Friedman JM,Stern SA

Published figure using Phospho-S6 (Ser244, Ser247) polyclonal antibody (Product # 44-923G) in Immunoprecipitation

Mon Sep 07 00:00:00 UTC 2020

Representation of Olfactory Information in Organized Active Neural Ensembles in the Hypothalamus.

Cell reports

Carvalho VMA,Nakahara TS,Souza MAA,Cardozo LM,Trintinalia GZ,Pissinato LG,Venancio JO,Stowers L,Papes F

44-923G was used in In Situ Hybridization (ISH) to study how a wide range of chemosignals form a discernible map of olfactory information in the ventromedial nucleus of the hypothalamus (VMH) and show how different stimuli entail distinct active neural ensembles.

Tue Aug 25 00:00:00 UTC 2020

Semiochemical responsive olfactory sensory neurons are sexually dimorphic and plastic.

eLife

Vihani A,Hu XS,Gundala S,Koyama S,Block E,Matsunami H

44-923G was used in Immunohistochemistry (Frozen) to generate a robust sexually dimorphic phenotype in the main olfactory epithelium.

Tue Nov 24 00:00:00 UTC 2020

A Role of Drd2 Hippocampal Neurons in Context-Dependent Food Intake.

Neuron

Azevedo EP,Pomeranz L,Cheng J,Schneeberger M,Vaughan R,Stern SA,Tan B,Doerig K,Greengard P,Friedman JM

44-923G was used in Immunohistochemistry-immunofluorescence to study neuronal regulation of food intake.

Wed May 22 00:00:00 UTC 2019

Behavioral evolution contributes to hindbrain diversification among Lake Malawi cichlid fish.

Scientific reports

York RA,Byrne A,Abdilleh K,Patil C,Streelman T,Finger TE,Fernald RD

44-923G was used in Immunohistochemistry to show that the evolution of a courtship behavior in Malawi cichlid fish is associated with rapid, extensive, and specific diversification of orosensory, gustatory centers in the hindbrain.

Fri Dec 27 00:00:00 UTC 2019

A Population Representation of Absolute Light Intensity in the Mammalian Retina.

Cell

Milner ES,Do MTH

44-923G was used in Immunohistochemistry-immunofluorescence to demonstrate that individual intrinsically photosensitive retinal ganglion cells (ipRGCs) in the mammalian retina operate over a narrow range, limited by depolarization block; the population covers a broad range of irradiances with flexibility and efficiency.

Thu Nov 02 00:00:00 UTC 2017

Dense Intra-adipose Sympathetic Arborizations Are Essential for Cold-Induced Beiging of Mouse White Adipose Tissue.

Cell metabolism

Jiang H,Ding X,Cao Y,Wang H,Zeng W

44-923G was used in Immunohistochemistry to examine the neural arborisations in mouse inguinal white adipose tissue at single-fibre resolution.

Tue Oct 03 00:00:00 UTC 2017

Olfactory receptor accessory proteins play crucial roles in receptor function and gene choice.

eLife

Sharma R,Ishimaru Y,Davison I,Ikegami K,Chien MS,You H,Chi Q,Kubota M,Yohda M,Ehlers M,Matsunami H

44923G was used in immunohistochemistry - frozen section to uncover the role of the receptor transporting protein family in G protein-coupled olfactory receptor expression and trafficking in olfactory sensory neurons

Mon Mar 06 00:00:00 UTC 2017

Warm-Sensitive Neurons that Control Body Temperature.

Cell

Tan CL,Cooke EK,Leib DE,Lin YC,Daly GE,Zimmerman CA,Knight ZA

44923G was used in immunohistochemistry - free floating to discover warm-sensitive neurons within the preoptic hypothalamus that regulate the response to heat

Thu Sep 22 00:00:00 UTC 2016

Molecular profiling of activated neurons by phosphorylated ribosome capture.

Cell

Knight ZA,Tan K,Birsoy K,Schmidt S,Garrison JL,Wysocki RW,Emiliano A,Ekstrand MI,Friedman JM

44-923G was used in immunocytochemistry to develop methods to capture of cell-type-specific transcripts using neurons.

Wed Nov 21 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 40S ribosomal protein S6; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; RP11-513M16.6; RS6; Small ribosomal subunit protein eS6

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Gene Aliases: OK/SW-cl.2; RPS6; S6

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UniProt ID: (Human) P62753

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Entrez Gene ID: (Human) 6194

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Function(s)

structural constituent of ribosome protein binding protein kinase binding poly(A) RNA binding

Process(es)

G1/S transition of mitotic cell cycle nuclear-transcribed mRNA catabolic process, nonsense-mediated decay placenta development T cell proliferation involved in immune response rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane activation-induced cell death of T cells mitotic nuclear division mitotic cell cycle checkpoint gastrulation viral transcription oogenesis stage TOR signaling T cell differentiation in thymus ribosomal small subunit biogenesis glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process erythrocyte development

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Phospho-S6 (Ser244, Ser247) Polyclonal Antibody

Phospho-S6 (Ser244, Ser247) Antibody (44-923G) in WB

Product Details

44-923G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

In Situ Hybridization (ISH) (ISH)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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