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Invitrogen
Phospho-SGK1 (Ser422) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-SGK1 (Ser422) Antibody (44-1264G) in IHC (P)
Immunohistochemistry analysis of SGK1 (PS422) showing staining in the cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperat... View More
Product Details
44-1264G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SGK1 that contains serine 422.
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533593
Target Information
SGK (SGK1) is a serine/threonine kinase that may be a transcriptional target for p38/MAPK in hepatoma cells. SGK plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: serine/threonine protein kinase SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1; Sgk1 variant i3
Gene Aliases: SGK; SGK1
UniProt ID: (Human) O00141
Entrez Gene ID: (Human) 6446
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
calcium channel regulator activity
protein binding
ATP binding
potassium channel regulator activity
sodium channel regulator activity
chloride channel regulator activity
3-phosphoinositide-dependent protein kinase binding
cofactor binding
tau protein binding
regulation of cell growth
protein phosphorylation
sodium ion transport
cellular sodium ion homeostasis
apoptotic process
response to stress
cellular response to DNA damage stimulus
microtubule depolymerization
long-term memory
regulation of blood pressure
visual learning
positive regulation of sodium ion transport
peptidyl-serine phosphorylation
positive regulation of cell growth
regulation of cell migration
negative regulation of microtubule polymerization
positive regulation of transporter activity
ion transmembrane transport
intracellular signal transduction
regulation of cell proliferation
regulation of apoptotic process
negative regulation of apoptotic process
glucocorticoid mediated signaling pathway
neuron projection morphogenesis
positive regulation of dendrite morphogenesis
regulation of catalytic activity
regulation of sequence-specific DNA binding transcription factor activity
regulation of cell cycle
regulation of gastric acid secretion
renal sodium ion absorption
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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