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Invitrogen
Phospho-SIK1 (Thr182) Polyclonal Antibody
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Phospho-SIK1 (Thr182) Antibody (PA5-105918) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-SIK1 (Thr182) in human lung cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-SIK1 (Thr182) polyclonal antibody (Product # PA5-105918) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105918
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SIK1(Accession P57059), corresponding to amino acid residues around phosphorylated Thr182.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2817317
Product Specific Information
Antibody detects endogenous levels of SIK only when phosphorylated at Thr182.
Target Information
SIK1 may mediate the physiological effects of LKB1, including its tumor suppressor function. SIK1 is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4, and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HRT-20; Myocardial SNF1-like kinase; Probable serine/threonine protein kinase SNF1LK (HRT-20) (Myocardial SNF1-like kinase); Protein kinase KID2; Salt-inducible kinase 1; salt-inducible protein kinase 1; serine/threonine protein kinase; Serine/threonine-protein kinase SIK1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK; SIK-1; SNF1-like kinase
Gene Aliases: EIEE30; Kid2; MSK; SIK; SIK1; SNF1LK
UniProt ID: (Human) P57059, (Mouse) Q60670, (Rat) Q9R1U5
Entrez Gene ID: (Human) 150094, (Mouse) 17691, (Rat) 59329
magnesium ion binding
protein serine/threonine kinase activity
protein binding
ATP binding
cAMP response element binding protein binding
protein kinase binding
14-3-3 protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
histone deacetylase binding
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
regulation of sodium ion transport
protein phosphorylation
cell cycle
multicellular organism development
regulation of mitotic cell cycle
regulation of myotube differentiation
negative regulation of triglyceride biosynthetic process
phosphorylation
cell differentiation
negative regulation of CREB transcription factor activity
intracellular signal transduction
entrainment of circadian clock by photoperiod
anoikis
negative regulation of gluconeogenesis
rhythmic process
cardiac muscle cell differentiation
regulation of cell differentiation
protein autophosphorylation
positive regulation of anoikis
cellular response to hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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