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Fabgennix
Phospho-SPT5 (Thr794) Polyclonal Antibody
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Product Details
PSPT5-140AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phosphorylated synthetic peptide corresponding to amino acids within region 760-810 on mouse SPT5 protein.
Conjugate
Form
Liquid
Concentration
0.5-1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer with 20% glycerol, 0.5% BSA
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Quantity: 100 µg IgG per vial
Target Information
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DRB sensitivity-inducing factor large subunit; DSIF large subunit; suppressor of Ty 5 homolog; Transcription elongation factor SPT5
Gene Aliases: AL033283; AL033321; AU019549; Spt5; Supt5; Supt5h
UniProt ID: (Mouse) O55201
Entrez Gene ID: (Mouse) 20924
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
DNA-templated transcription, elongation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
positive regulation of macroautophagy
regulation of DNA-templated transcription, elongation
negative regulation of DNA-templated transcription, elongation
positive regulation of DNA-templated transcription, elongation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of mRNA polyadenylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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