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          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), eBioscience™

          5 References
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          Cite Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), eBioscience™

          • Antibody Testing Data (1)
          Phospho-SRC (Tyr418) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-SRC (Tyr418) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Phospho-SRC (Tyr418) Antibody (14-9034-82) in WB

          Immunoblotting of reduced lysates from Jurkat T cells left untreated (lane 1), treated with hydrogen peroxide-activated sodium pervanadate (lane 2), or treated with staurosporine (lane 3) using 5 µg/mL of Anti-Human/Mouse phospho-Src (Y418) Purified. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-SRC (Tyr418) Antibody in Western Blot (WB)

          Product Details

          14-9034-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-Dependent
          -

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG2b, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          SC1T2M3

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • eFluor 660
          • PE 
          • PerCP-eFluor 710
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2572916

          Product Specific Information

          Description: This SC1T2M3 monoclonal antibody recognizes human and mouse Src tyrosine kinase (also known as ASV, c-src, c-SRC, p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1) when phosphorylated on tyrosine 418 (Y418). Autophosphorylation of Src at Y418 in the catalytic domain is required for full catalytic activity of this kinase. Src is a non-receptor tyrosine kinase involved in signal transduction in numerous biological systems and is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Aberrant Src activity has been implicated in the development of numerous types of cancer. Due to the sequence homology surrounding Src Y418, this SC1T2M3 clone is predicted to cross-react with many Src family kinases including Src, Lck, Fyn, and Lyn.

          Specificity of this SC1T2M3 clone was determined by ELISA, flow cytometry, and western blotting.

          Applications Reported: This SC1T2M3 antibody has been reported for use in western blotting. (Fluorochrome-conjugated SC1T2M3 is recommended for use in intracellular flow cytometry.).

          Applications Tested: This SC1T2M3 antibody has been tested by immunoblotting of staurosporine-treated Jurkat cells. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60c-src; pp60src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; RP5-823N20.1; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

          View more View less

          Gene Aliases: ASV; AW259666; c-SRC; p60-Src; pp60c-src; SRC; SRC1; THC6

          View more View less

          UniProt ID: (Human) P12931, (Mouse) P05480

          View more View less

          Entrez Gene ID: (Human) 6714, (Mouse) 20779

          View more View less

          Function(s)
          protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity SH3/SH2 adaptor activity protein kinase C binding receptor binding insulin receptor binding integrin binding protein binding ATP binding protein C-terminus binding kinase activity enzyme binding kinase binding heme binding estrogen receptor binding SH2 domain binding ion channel binding ephrin receptor binding phosphoprotein binding hormone receptor binding growth factor receptor binding scaffold protein binding cadherin binding involved in cell-cell adhesion nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding protein complex binding cell adhesion molecule binding
          Process(es)
          immune system process protein phosphorylation cell cycle cell adhesion epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway cell proliferation positive regulation of gene expression regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of glucose metabolic process positive regulation of smooth muscle cell migration phosphorylation cell migration peptidyl-serine phosphorylation peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion cell differentiation forebrain development positive regulation of protein autophosphorylation activation of protein kinase B activity negative regulation of protein homooligomerization regulation of intracellular estrogen receptor signaling pathway adherens junction organization substrate adhesion-dependent cell spreading cellular response to reactive oxygen species intracellular signal transduction osteoclast development cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation regulation of cell proliferation positive regulation of apoptotic process negative regulation of apoptotic process stress fiber assembly negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of protein binding positive regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity transcytosis innate immune response bone resorption positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell adhesion negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of insulin receptor signaling pathway protein autophosphorylation neurotrophin TRK receptor signaling pathway oogenesis positive regulation of cytokine secretion progesterone receptor signaling pathway positive regulation of protein transport regulation of cell cycle negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly angiotensin-activated signaling pathway involved in heart process positive regulation of canonical Wnt signaling pathway positive regulation of intracellular signal transduction positive regulation of lamellipodium morphogenesis positive regulation of DNA biosynthetic process regulation of early endosome to late endosome transport negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway regulation of caveolin-mediated endocytosis stimulatory C-type lectin receptor signaling pathway signal transduction signal complex assembly central nervous system development response to mechanical stimulus response to virus response to acidic pH positive regulation of platelet-derived growth factor receptor signaling pathway single organismal cell-cell adhesion platelet activation intracellular estrogen receptor signaling pathway T cell costimulation protein destabilization response to nutrient levels negative regulation of telomere maintenance via telomerase cellular response to insulin stimulus positive regulation of integrin activation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway response to drug response to hydrogen peroxide regulation of vascular permeability regulation of bone resorption platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration positive regulation of small GTPase mediated signal transduction response to mineralocorticoid response to electrical stimulus negative regulation of telomerase activity cellular response to lipopolysaccharide cellular response to fatty acid cellular response to hypoxia regulation of podosome assembly positive regulation of protein serine/threonine kinase activity
          It has to be done as per old AB suggested Products section.
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