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Invitrogen
Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), eFluor™ 450, eBioscience™
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FIGURE: 1 / 3
Phospho-STAT1 (Tyr701) Antibody (48-9008-42) in Flow
Intracellular staining of unstimulated (orange histogram) or 15-minute IFN gamma-stimulated (purple histogram) normal human peripheral blood cells with Anti-Human phospho-STAT1 (Y701) eFluor® 450 using the Fixation/Methanol Protocol. CD4-low monocytes were used for analysis.
Product Details
48-9008-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
KIKSI0803
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574119
Product Specific Information
Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements.
Specificity of this KIKSI0803 clone was determined by ELISA, flow cytometry, and western blotting.
Applications Reported:This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This KIKSI0803 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.
eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT-1; stat1 alpha; Transcription factor ISGF-3 components p91/p84
Gene Aliases: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91
UniProt ID: (Human) P42224
Entrez Gene ID: (Human) 6772
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter sequence-specific
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
signal transducer activity
tumor necrosis factor receptor binding
protein binding
enzyme binding
nuclear hormone receptor binding
identical protein binding
protein homodimerization activity
cadherin binding involved in cell-cell adhesion
negative regulation of transcription from RNA polymerase II promoter
negative regulation of endothelial cell proliferation
positive regulation of mesenchymal cell proliferation
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
transcription, DNA-templated
apoptotic process
JAK-STAT cascade
blood circulation
macrophage derived foam cell differentiation
negative regulation of angiogenesis
tumor necrosis factor-mediated signaling pathway
response to cytokine
response to interferon-beta
cellular response to interferon-beta
regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
response to peptide hormone
endothelial cell migration
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation by virus of viral protein levels in host cell
positive regulation of smooth muscle cell proliferation
response to cAMP
defense response to virus
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
regulation of type I interferon-mediated signaling pathway
renal tubule development
cellular response to organic cyclic compound
metanephric mesenchymal cell proliferation involved in metanephros development
metanephric mesenchymal cell differentiation
negative regulation of metanephric nephron tubule epithelial cell differentiation
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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