Invitrogen

Phospho-STAT1 (Tyr701) Monoclonal Antibody (S.213.5)

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2 Published Figures
5 References
View all (90) STAT1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15071

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:1,600
-

Immunocytochemistry (ICC/IF)

1:200-1:800
View 2 publications 2 publications

Flow Cytometry (Flow)

1:100-1:400
-

Immunoprecipitation (IP)

1:100
-

ChIP assay (ChIP)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

S.213.5

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pTyr701 of human Stat1
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

59 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10983352

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with the corresponding phospho-tyrosines of other Stat proteins.

Target Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT-1; stat1 alpha; Transcription factor ISGF-3 components p91/p84

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Gene Aliases: 2010005J02Rik; AA408197; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91

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UniProt ID: (Human) P42224

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Entrez Gene ID: (Human) 6772, (Mouse) 20846

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific double-stranded DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity tumor necrosis factor receptor binding protein binding enzyme binding nuclear hormone receptor binding identical protein binding protein homodimerization activity cadherin binding involved in cell-cell adhesion DNA binding CCR5 chemokine receptor binding sequence-specific DNA binding protein phosphatase 2A binding
Process(es)
negative regulation of endothelial cell proliferation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction JAK-STAT cascade response to nutrient blood circulation positive regulation of cell proliferation response to mechanical stimulus response to bacterium negative regulation of angiogenesis cytokine-mediated signaling pathway lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide cellular response to insulin stimulus tumor necrosis factor-mediated signaling pathway response to cytokine negative regulation of macrophage fusion cellular response to interferon-beta response to drug negative regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA response to peptide hormone positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation by virus of viral protein levels in host cell positive regulation of smooth muscle cell proliferation response to cAMP interferon-gamma-mediated signaling pathway type I interferon signaling pathway renal tubule development cellular response to lipopolysaccharide negative regulation of transcription from RNA polymerase II promoter positive regulation of mesenchymal cell proliferation negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis apoptotic process macrophage derived foam cell differentiation response to interferon-beta regulation of apoptotic process endothelial cell migration defense response to virus regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway cellular response to organic cyclic compound metanephric mesenchymal cell proliferation involved in metanephros development metanephric mesenchymal cell differentiation negative regulation of metanephric nephron tubule epithelial cell differentiation cell-cell adhesion
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