Bioss

Phospho-STAT3 (Tyr705) Polyclonal Antibody

View all (107) STAT3 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

BS-1658R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:400
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1 µg/test
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Chicken, Human, Mouse, Pig, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: acute phase response factor; Acute-phase response factor; DNA-binding protein APRF; FLJ20882; MGC16063; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 (acute-phase response factor); STAT

View more View less

Gene Aliases: 1110034C02Rik; ADMIO; ADMIO1; APRF; AW109958; HIES; RCJMB04_38l20; STAT3

View more View less

UniProt ID: (Pig) Q19S50, (Chicken) Q6DV79, (Human) P40763, (Rat) P52631, (Mouse) P42227

View more View less

Entrez Gene ID: (Pig) 733648, (Rabbit) 100348778, (Chicken) 420027, (Human) 6774, (Rat) 25125, (Mouse) 20848

View more View less

Function(s)
RNA polymerase II repressing transcription factor binding DNA binding signal transducer activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding calcium ion binding transcription factor binding protein kinase binding protein phosphatase binding sequence-specific DNA binding transcription regulatory region DNA binding protein dimerization activity RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding chromatin DNA binding CCR5 chemokine receptor binding glucocorticoid receptor binding identical protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding
Process(es)
temperature homeostasis eye photoreceptor cell differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein import into nucleus cell proliferation phosphorylation stem cell population maintenance sexual reproduction regulation of multicellular organism growth glucose homeostasis eating behavior positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter astrocyte differentiation radial glial cell differentiation JAK-STAT cascade involved in growth hormone signaling pathway interleukin-6-mediated signaling pathway negative regulation of neuron migration acute-phase response JAK-STAT cascade aging negative regulation of cell proliferation response to organic substance negative regulation of hydrogen peroxide biosynthetic process response to organic cyclic compound intracellular receptor signaling pathway response to estradiol cellular response to hormone stimulus response to cytokine response to drug response to peptide hormone response to ethanol negative regulation of glycolytic process regulation of mitochondrial membrane permeability growth hormone receptor signaling pathway negative regulation of cell death negative regulation of neuron death positive regulation of growth factor dependent skeletal muscle satellite cell proliferation positive regulation of ATP biosynthetic process negative regulation of transcription from RNA polymerase II promoter movement of cell or subcellular component signal transduction nervous system development positive regulation of gene expression viral process cytokine-mediated signaling pathway leptin-mediated signaling pathway somatic stem cell population maintenance miRNA mediated inhibition of translation positive regulation of tyrosine phosphorylation of Stat3 protein negative regulation of apoptotic process cellular response to leptin stimulus response to leptin regulation of cell cycle regulation of feeding behavior cellular response to organic cyclic compound energy homeostasis positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of metalloendopeptidase activity positive regulation of gene silencing by miRNA transcription, DNA-templated
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support