Invitrogen

Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE, eBioscience™

Phospho-STAT5 (Tyr694) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 9

Phospho-STAT5 (Tyr694) Antibody (12-9010-42) in Flow

LEFT: Intracellular staining of untreated (orange histogram) or 15-minute IL-2-treated (purple histogram) human Th2-polarized CD4+ with Anti-Human/Mouse phospho-STAT5 (Y694) PE. Cells in the lymphocyte gate were used for analysis. RIGHT: Intracellular staining of untreated (orange histogram) or 15-minute GM-CSF-treated (purple histogram) mouse thioglycolate-elicited peritoneal exudate cells with Anti-Human/... View More

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Product Details

12-9010-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SRBCZX

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572671

Product Specific Information

Description: This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to cytokines that signal through the common gamma chain such as IL-2, IL-7, and IL-15, STAT5 is phosphorylated on tyrosine 694 by JAK1 and JAK3. Cytokines such as IL-3, IL-5, and GM-CSF that signal via the common beta chain induce STAT5 phosphorylation on tyrosine 694 by JAK 2. Phosphorylation of STAT5 on tyrosine 694 is essential for STAT5 dimer formation, nuclear translocation, and DNA binding activity.

Specificity of this SRBCZX clone was determined by ELISA and flow cytometry.

Applications Reported:This SRBCZX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SRBCZX antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Establishing a novel and sensitive assay for bioactivity determination of anti-CD25 antibodies.

Heliyon

Duan M,Yu C,Yang Y,Fu Z,Liu C,Du J,Li M,Guo S,Yu X,Xu G,Mei Y,Wang L

Published figure using Phospho-STAT5 (Tyr694) monoclonal antibody (Product # 12-9010-42) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2023

Interleukin-23 receptor defines T helper 1-like regulatory T cells in oral squamous cell carcinoma.

Immunity, inflammation and disease

Li W,An N,Wang M,Liu X,Mei Z

12-9010-42 was used in Flow cytometry/Cell sorting to unveil Treg heterogeneity, thus deepening the understanding of Treg biology and tumor immunity in OSCC.

Thu Dec 01 00:00:00 UTC 2022

GM-CSF impairs erythropoiesis by disrupting erythroblastic island formation via macrophages.

Journal of translational medicine

Cao W,Fan W,Wang F,Zhang Y,Wu G,Shi X,Shi JX,Gao F,Yan M,Guo R,Li Y,Li W,Du C,Jiang Z

12-9010-42 was used in Flow Cytometry to document that GM-CSF impairs EBI formation in mice and humans.

Mon Jan 03 00:00:00 UTC 2022

GM-CSF impairs erythropoiesis by disrupting erythroblastic island formation via macrophages.

Journal of translational medicine

Cao W,Fan W,Wang F,Zhang Y,Wu G,Shi X,Shi JX,Gao F,Yan M,Guo R,Li Y,Li W,Du C,Jiang Z

12-9010-42 was used in Flow Cytometry to document that GM-CSF impairs EBI formation in mice and humans.

Mon Jan 03 00:00:00 UTC 2022

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

12-9010-42 was used in Flow Cytometry to show increased formation of Tfh precursors (pre-Tfh) but no associated increase in germinal centre (GC)-Tfh cells in aged mice, suggesting age-driven promotion of only early Tfh cell differentiation.

Fri Jan 01 00:00:00 UTC 2021

Aged neutrophils form mitochondria-dependent vital NETs to promote breast cancer lung metastasis.

Journal for immunotherapy of cancer

Yang C,Wang Z,Li L,Zhang Z,Jin X,Wu P,Sun S,Pan J,Su K,Jia F,Zhang L,Wang H,Yu X,Shao X,Wang K,Qiu F,Yan J,Huang J

12-9010-42 was used in Flow Cytometry to investigate the existence and biological function of a rarely delved subset of neutrophils, named as tumor-associated aged neutrophils (Naged, CXCR4+CD62Llow), involved in premetastatic niche formation during breast cancer metastasis.

Fri Oct 01 00:00:00 UTC 2021

Aged neutrophils form mitochondria-dependent vital NETs to promote breast cancer lung metastasis.

Journal for immunotherapy of cancer

Yang C,Wang Z,Li L,Zhang Z,Jin X,Wu P,Sun S,Pan J,Su K,Jia F,Zhang L,Wang H,Yu X,Shao X,Wang K,Qiu F,Yan J,Huang J

Fri Oct 01 00:00:00 UTC 2021

Long-Term Programming of CD8 T Cell Immunity by Perinatal Exposure to Glucocorticoids.

Cell

Hong JY,Lim J,Carvalho F,Cho JY,Vaidyanathan B,Yu S,Annicelli C,Ip WKE,Medzhitov R

12-9010 was used in Flow cytometry/Cell sorting to demonstrate that perinatal stress can have long-term consequences on CD8 T cell immunity by altering HPA axis activity.

Thu Mar 05 00:00:00 UTC 2020

ILC3 deficiency and generalized ILC abnormalities in DOCK8-deficient patients.

Allergy

Eken A,Cansever M,Okus FZ,Erdem S,Nain E,Azizoglu ZB,Haliloglu Y,Karakukcu M,Ozcan A,Devecioglu O,Aksu G,Arikan Ayyildiz Z,Topal E,Karakoc Aydiner E,Kiykim A,Metin A,Cipe F,Kaya A,Artac H,Reisli I,Guner SN,Uygun V,Karasu G,Dönmez Altuntas H,Canatan H,Oukka M,Ozen A,Chatila TA,Keles S,Baris S,Unal E,Patiroglu T

12-9010 was used in Flow cytometry/Cell sorting to sought to test whether DOCK8 is required for the function and maintenance of ILC subsets in humans.

Wed Apr 01 00:00:00 UTC 2020

SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.

Cancer immunology research

Hu M,Lu Y,Qi Y,Zhang Z,Wang S,Xu Y,Chen F,Tang Y,Chen S,Chen M,Du C,Shen M,Wang F,Su Y,Deng Y,Wang J

12-9010-42 was used in Flow Cytometry to study the role of SRC-3 as a coactivator of T-bet in natural killer-cell biology and indicate that targeting SRC-3 may be a promising strategy to increase the tumor surveillance function of natural killer cells.

Tue Sep 01 00:00:00 UTC 2020

Novel cytokine-antibody fusion protein, N-820, to enhance the functions of ex vivo expanded natural killer cells against Burkitt lymphoma.

Journal for immunotherapy of cancer

Chu Y,Nayyar G,Kham Su N,Rosenblum JM,Soon-Shiong P,Lee J,Safrit JT,Barth M,Lee D,Cairo MS

12-9010 was used in Flow cytometry/Cell sorting to provide the rationale for the development of a clinical trial of N-820 alone or in combination with endogenous or ex vivo expanded NK cells in patients with CD20+ B-NHL failing prior rituximab containing chemoimmunotherapy regimens.

Thu Oct 01 00:00:00 UTC 2020

Receptor-mediated dimerization of JAK2 FERM domains is required for JAK2 activation.

eLife

Ferrao RD,Wallweber HJ,Lupardus PJ

Published figure using Phospho-STAT5 (Tyr694) monoclonal antibody (Product # 12-9010-42) in Flow Cytometry

Wed Jul 25 00:00:00 UTC 2018

CISH promoter polymorphism effects on T cell cytokine receptor signaling and type 1 diabetes susceptibility.

Molecular and cellular pediatrics

Seyfarth J,Ahlert H,Rosenbauer J,Baechle C,Roden M,Holl RW,Mayatepek E,Meissner T,Jacobsen M

12-9010 was used in Flow cytometry/Cell sorting to analyzed allele frequencies of three CISH SNPs (i.e., rs809451, rs414171, rs2239751) in a study of T1D patients (n = 260, onset age 5 years, duration > 10 years).

Tue Feb 06 00:00:00 UTC 2018

Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis.

PLoS pathogens

Lundtoft C,Afum-Adjei Awuah A,Rimpler J,Harling K,Nausch N,Kohns M,Adankwah E,Lang F,Olbrich L,Mayatepek E,Owusu-Dabo E,Jacobsen M

12-9010 was used in Flow cytometry/Cell sorting to establish the causing of impaired T cell sensitivity to IL-7 in tuberculosis patients to be diminished soluble IL-7R and increases IL-7 plasma concentrations.

Thu Jun 01 00:00:00 UTC 2017

Erythropoietin enhances Kupffer cell number and activity in the challenged liver.

Scientific reports

Gilboa D,Haim-Ohana Y,Deshet-Unger N,Ben-Califa N,Hiram-Bab S,Reuveni D,Zigmond E,Gassmann M,Gabet Y,Varol C,Neumann D

Published figure using Phospho-STAT5 (Tyr694) monoclonal antibody (Product # 12-9010-42) in Flow Cytometry

Mon Sep 04 00:00:00 UTC 2017

Environmentally relevant concentrations of arsenite and monomethylarsonous acid inhibit IL-7/STAT5 cytokine signaling pathways in mouse CD3+CD4-CD8- double negative thymus cells.

Toxicology letters

Xu H,Lauer FT,Liu KJ,Hudson LG,Burchiel SW

12-9010 was used in Western Blotting to suggest that arsenic at environmentally-relevant doses suppresses early T-cell development through inhibition of IL-7 signaling.

Fri Apr 15 00:00:00 UTC 2016

Environmentally relevant concentrations of arsenite and monomethylarsonous acid inhibit IL-7/STAT5 cytokine signaling pathways in mouse CD3+CD4-CD8- double negative thymus cells.

Toxicology letters

Xu H,Lauer FT,Liu KJ,Hudson LG,Burchiel SW

12-9010 was used in Western Blotting to suggest that arsenic at environmentally-relevant doses suppresses early T-cell development through inhibition of IL-7 signaling.

Fri Apr 15 00:00:00 UTC 2016

Monomethylarsonous acid (MMA+3) Inhibits IL-7 Signaling in Mouse Pre-B Cells.

Toxicological sciences : an official journal of the Society of Toxicology

Ezeh PC,Xu H,Lauer FT,Liu KJ,Hudson LG,Burchiel SW

12-9010 was used in Flow cytometry/Cell sorting to investigate the role of monomethylarsonous acid in pre-B cells.

Mon Feb 01 00:00:00 UTC 2016

Expression and function of hematopoiesis-stimulating factor receptors on the GPI^- and GPI⁺ hematopoietic stem cells of patients with paroxysmal nocturnal hemoglobinuria/aplastic anemia syndrome.

Experimental and therapeutic medicine

Fu R,Ding SX,Liu YI,Li LJ,Liu H,Wang HL,Zhang T,Shao ZH

Published figure using Phospho-STAT5 (Tyr694) monoclonal antibody (Product # 12-9010-42) in Flow Cytometry

Sun May 01 00:00:00 UTC 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using Phospho-STAT5 (Tyr694) monoclonal antibody (Product # 12-9010-42) in Flow Cytometry

Tue Aug 09 00:00:00 UTC 2016

Combination of imatinib with CXCR4 antagonist BKT140 overcomes the protective effect of stroma and targets CML in vitro and in vivo.

Molecular cancer therapeutics

Beider K,Darash-Yahana M,Blaier O,Koren-Michowitz M,Abraham M,Wald H,Wald O,Galun E,Eizenberg O,Peled A,Nagler A

12-9010 was used in Flow cytometry/Cell sorting to indicate the importance of CXCR4/CXCL12 axis in CML growth and CML-BM stroma interaction.

Thu May 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Mammary gland factor; mammary gland factor STAT5A; Signal transducer and activator of transcription 5A; STAT; STAT5B

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Gene Aliases: AA959963; MGF; Mpf; STAT5; STAT5A

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UniProt ID: (Human) P42229, (Mouse) P42230

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Entrez Gene ID: (Human) 6776, (Mouse) 20850

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding RNA polymerase II core promoter sequence-specific DNA binding double-stranded DNA binding protein phosphatase binding sequence-specific DNA binding

Process(es)

Performance Guarantee

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Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE, eBioscience™

Phospho-STAT5 (Tyr694) Antibody (12-9010-42) in Flow

Product Details

12-9010-42

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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