Invitrogen

Phospho-Syk (Tyr525, Tyr526) Monoclonal Antibody (F.724.5)

Phospho-Syk (Tyr525, Tyr526) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Syk (Tyr525, Tyr526) Antibody (MA5-14918) in ICC/IF

Immunofluorescence analysis of Phospho-Syk (Tyr525, Tyr526) was performed using log phase Ramos anti-Human IgM (12 µg/mL, 2 min). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Syk (Tyr525, Tyr526) Monoclonal Antibody (F.724.5) (Product # MA5-14918,... View More

Phospho-Syk (Tyr525, Tyr526) Antibody in Western Blot (WB)

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Product Details

MA5-14918

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:100-1:400

Flow Cytometry (Flow)

1:200-1:800

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.724.5

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pTyr525/526 of human Syk

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

90 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10989513

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other tyrosine-phosphorylated protein tyrosine kinases.

Target Information

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Syk inhibitors protect against microglia-mediated neuronal loss in culture.

Frontiers in aging neuroscience

Birkle TJY,Brown GC

MA5-14918 was used in Western Blot to test whether Syk inhibitors can prevent microglia-dependent neurodegeneration induced by lipopolysaccharide (LPS) in primary neuron-glia cultures.

Wed Feb 14 00:00:00 UTC 2024

Antitumor Immune Mechanisms of the Anti-Complement Factor H Antibody GT103.

Molecular cancer therapeutics

Bushey RT,Saxena R,Campa MJ,Gottlin EB,He YW,Patz EF

MA5-14918 was used in Western Blotting to demonstrate that GT103 causes B-cell activation in vitro and in vivo, and that GT103 antitumor activity in vivo is B-cell dependent.

Thu Jun 01 00:00:00 UTC 2023

Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway.

Aging cell

Jung ES,Suh K,Han J,Kim H,Kang HS,Choi WS,Mook-Jung I

MA5-14918 was used in Western Blot to suggest that repurposing of FA for inhibiting microglial activation of the NLRP3 inflammasome is a potential treatment for AD.

Sun May 01 00:00:00 UTC 2022

LILRB2-mediated TREM2 signaling inhibition suppresses microglia functions.

Molecular neurodegeneration

Zhao P,Xu Y,Jiang LL,Fan X,Ku Z,Li L,Liu X,Deng M,Arase H,Zhu JJ,Huang TY,Zhao Y,Zhang C,Xu H,Tong Q,Zhang N,An Z

MA5-14918 was used in Western Blotting to reveal the molecular mechanisms of LILRB2-mediated inhibition of TREM2 signaling in microglia and demonstrate a novel approach of enhancing TREM2-mediated microglia functions by blocking LILRB2-ligand interactions.

Sat Jun 18 00:00:00 UTC 2022

PLCγ2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages.

Scientific reports

Obst J,Hall-Roberts HL,Smith TB,Kreuzer M,Magno L,Di Daniel E,Davis JB,Mead E

Published figure using Phospho-Syk (Tyr525, Tyr526) monoclonal antibody (Product # MA5-14918) in Western Blot

Wed Oct 06 00:00:00 UTC 2021

Molecular basis of carrageenan-induced cytokines production in macrophages.

Cell communication and signaling : CCS

Lopes AH,Silva RL,Fonseca MD,Gomes FI,Maganin AG,Ribeiro LS,Marques LMM,Cunha FQ,Alves-Filho JC,Zamboni DS,Lopes NP,Franklin BS,Gombault A,Ramalho FS,Quesniaux VFJ,Couillin I,Ryffel B,Cunha TM

MA5-14918 was used in Western Blotting to unravel the molecular basis for Cg-induced macrophages production of biological active IL-1β.

Mon Sep 07 00:00:00 UTC 2020

Molecular basis of carrageenan-induced cytokines production in macrophages.

Cell communication and signaling : CCS

Lopes AH,Silva RL,Fonseca MD,Gomes FI,Maganin AG,Ribeiro LS,Marques LMM,Cunha FQ,Alves-Filho JC,Zamboni DS,Lopes NP,Franklin BS,Gombault A,Ramalho FS,Quesniaux VFJ,Couillin I,Ryffel B,Cunha TM

MA5-14918 was used in Western Blotting to unravel the molecular basis for Cg-induced macrophages production of biological active IL-1β.

Mon Sep 07 00:00:00 UTC 2020

Diabetic phenotype in mouse and humans reduces the number of microglia around β-amyloid plaques.

Molecular neurodegeneration

Natunen T,Martiskainen H,Marttinen M,Gabbouj S,Koivisto H,Kemppainen S,Kaipainen S,Takalo M,Svobodová H,Leppänen L,Kemiläinen B,Ryhänen S,Kuulasmaa T,Rahunen E,Juutinen S,Mäkinen P,Miettinen P,Rauramaa T,Pihlajamäki J,Haapasalo A,Leinonen V,Tanila H,Hiltunen M

MA5-14918 was used in Western Blotting to suggest that the diabetic phenotype in mice and humans mechanistically associates with abnormally reduced microglial responses to β-amyloid pathology and further suggest that AD and T2D share overlapping pathomechanisms, likely involving altered immune function in the brain.

Tue Nov 10 00:00:00 UTC 2020

Diabetic phenotype in mouse and humans reduces the number of microglia around β-amyloid plaques.

Molecular neurodegeneration

Tue Nov 10 00:00:00 UTC 2020

TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages.

Alzheimer's research & therapy

Hall-Roberts H,Agarwal D,Obst J,Smith TB,Monzón-Sandoval J,Di Daniel E,Webber C,James WS,Mead E,Davis JB,Cowley SA

MA5-14918 was used in Western Blotting to investigated cell phenotypes of the R47H mutant and TREM2 KO in a model of human microglia, and compared their transcriptional signatures, to determine the mechanism by which R47H TREM2 disrupts function.

Mon Nov 16 00:00:00 UTC 2020

TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages.

Alzheimer's research & therapy

Hall-Roberts H,Agarwal D,Obst J,Smith TB,Monzón-Sandoval J,Di Daniel E,Webber C,James WS,Mead E,Davis JB,Cowley SA

Mon Nov 16 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

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Gene Aliases: p72-Syk; SYK

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UniProt ID: (Human) P43405

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Entrez Gene ID: (Human) 6850

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity integrin binding protein binding ATP binding protein kinase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-Syk (Tyr525, Tyr526) Monoclonal Antibody (F.724.5)

Phospho-Syk (Tyr525, Tyr526) Antibody (MA5-14918) in ICC/IF

Product Details

MA5-14918

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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