Proteintech

Phospho-TDP43 (Ser409, Ser410) Monoclonal Antibody (1A2C1)

Phospho-TDP43 (Ser409, Ser410) Antibody in Western Blot (WB)

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Phospho-TDP43 (Ser409, Ser410) Antibody (66318-1-IG) in WB

Untreated and ethacrynic acid treated HeLa cells were subjected to SDS PAGE followed by western blot with 66318-1-IG (phospho (409/410)-TDP43 Antibody) at dilution of 1:850 incubated at room temperature for 1.5 hours.

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Product Details

66318-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1A2C1

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

66318-1-Ig is a phospho-dependent monoclonal antibody specifically recognizing phospho-TDP43 (409/410). It does not react with native TDP-43.

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomal TAR DNA binding protein 43 profile in canine model of amyotrophic lateral sclerosis: a preliminary study in developing blood-based biomarker for neurodegenerative diseases.

Annals of medicine

Pfeiffer P,Coates JR,Esqueda YM,Kennedy A,Getchell K,McLenon M,Kosa E,Agbas A

Fri Dec 01 00:00:00 UTC 2023

Casein Kinase 2 Mediates HIV- and Opioid-Induced Pathologic Phosphorylation of TAR DNA Binding Protein 43 in the Basal Ganglia.

ASN neuro

Ohene-Nyako M,Nass SR,Richard HT,Lukande R,Nicol MR,McRae M,Knapp PE,Hauser KF

Fri Mar 10 00:00:00 UTC 2023

Casein Kinase 2 Mediates HIV- and Opioid-Induced Pathologic Phosphorylation of TAR DNA Binding Protein 43 in the Basal Ganglia.

ASN neuro

Ohene-Nyako M,Nass SR,Richard HT,Lukande R,Nicol MR,McRae M,Knapp PE,Hauser KF

Fri Mar 10 00:00:00 UTC 2023

Casein Kinase 2 Mediates HIV- and Opioid-Induced Pathologic Phosphorylation of TAR DNA Binding Protein 43 in the Basal Ganglia.

ASN neuro

Ohene-Nyako M,Nass SR,Richard HT,Lukande R,Nicol MR,McRae M,Knapp PE,Hauser KF

Fri Mar 10 00:00:00 UTC 2023

Casein Kinase 2 Mediates HIV- and Opioid-Induced Pathologic Phosphorylation of TAR DNA Binding Protein 43 in the Basal Ganglia.

ASN neuro

Ohene-Nyako M,Nass SR,Richard HT,Lukande R,Nicol MR,McRae M,Knapp PE,Hauser KF

Fri Mar 10 00:00:00 UTC 2023

Myopathic changes caused by protein aggregates in adult-onset spinal muscular atrophy.

Neuropathology : official journal of the Japanese Society of Neuropathology

Yamashita S,Nagatoshi A,Takeuchi Y,Nishino I,Ueda M

Sun Oct 01 00:00:00 UTC 2023

Ex vivo MRI and histopathology detect novel iron-rich cortical inflammation in frontotemporal lobar degeneration with tau versus TDP-43 pathology.

NeuroImage. Clinical

Tisdall MD,Ohm DT,Lobrovich R,Das SR,Mizsei G,Prabhakaran K,Ittyerah R,Lim S,McMillan CT,Wolk DA,Gee J,Trojanowski JQ,Lee EB,Detre JA,Yushkevich P,Grossman M,Irwin DJ

Thu Mar 03 00:00:00 UTC 2022

Ex vivo MRI atlas of the human medial temporal lobe: characterizing neurodegeneration due to tau pathology.

Acta neuropathologica communications

Ravikumar S,Wisse LEM,Lim S,Ittyerah R,Xie L,Bedard ML,Das SR,Lee EB,Tisdall MD,Prabhakaran K,Lane J,Detre JA,Mizsei G,Trojanowski JQ,Robinson JL,Schuck T,Grossman M,Artacho-Pérula E,de Onzoño Martin MMI,Del Mar Arroyo Jiménez M,Muñoz M,Romero FJM,Del Pilar Marcos Rabal M,Sánchez SC,González JCD,de la Rosa Prieto C,Parada MC,Irwin DJ,Wolk DA,Insausti R,Yushkevich PA

Sun Oct 24 00:00:00 UTC 2021

Stress Induces Dynamic, Cytotoxicity-Antagonizing TDP-43 Nuclear Bodies via Paraspeckle LncRNA NEAT1-Mediated Liquid-Liquid Phase Separation.

Molecular cell

Wang C,Duan Y,Duan G,Wang Q,Zhang K,Deng X,Qian B,Gu J,Ma Z,Zhang S,Guo L,Liu C,Fang Y

Thu Aug 06 00:00:00 UTC 2020

Endoplasmic Reticulum Stress Signalling Induces Casein Kinase 1-Dependent Formation of Cytosolic TDP-43 Inclusions in Motor Neuron-Like Cells.

Neurochemical research

Hicks DA,Cross LL,Williamson R,Rattray M

Mon Jun 01 00:00:00 UTC 2020

SOD1 p.D12Y variant is associated with amyotrophic lateral sclerosis/distal myopathy spectrum.

European journal of neurology

Tasca G,Lattante S,Marangi G,Conte A,Bernardo D,Bisogni G,Mandich P,Zollino M,Ragozzino E,Udd B,Sabatelli M

Wed Jul 01 00:00:00 UTC 2020

The N Termini of TAR DNA-Binding Protein 43 (TDP43) C-Terminal Fragments Influence Degradation, Aggregation Propensity, and Morphology.

Molecular and cellular biology

Kasu YAT,Alemu S,Lamari A,Loew N,Brower CS

Mon Oct 01 00:00:00 UTC 2018

The N Termini of TAR DNA-Binding Protein 43 (TDP43) C-Terminal Fragments Influence Degradation, Aggregation Propensity, and Morphology.

Molecular and cellular biology

Kasu YAT,Alemu S,Lamari A,Loew N,Brower CS

Mon Oct 01 00:00:00 UTC 2018

The N Termini of TAR DNA-Binding Protein 43 (TDP43) C-Terminal Fragments Influence Degradation, Aggregation Propensity, and Morphology.

Molecular and cellular biology

Kasu YAT,Alemu S,Lamari A,Loew N,Brower CS

Mon Oct 01 00:00:00 UTC 2018

The N Termini of TAR DNA-Binding Protein 43 (TDP43) C-Terminal Fragments Influence Degradation, Aggregation Propensity, and Morphology.

Molecular and cellular biology

Kasu YAT,Alemu S,Lamari A,Loew N,Brower CS

Mon Oct 01 00:00:00 UTC 2018

Progressive ataxia of Charolais cattle highlights a role of KIF1C in sustainable myelination.

PLoS genetics

Duchesne A,Vaiman A,Frah M,Floriot S,Legoueix-Rodriguez S,Desmazières A,Fritz S,Beauvallet C,Albaric O,Venot E,Bertaud M,Saintilan R,Guatteo R,Esquerré D,Branchu J,Fleming A,Brice A,Darios F,Vilotte JL,Stevanin G,Boichard D,El Hachimi KH

Wed Aug 01 00:00:00 UTC 2018

Progressive ataxia of Charolais cattle highlights a role of KIF1C in sustainable myelination.

PLoS genetics

Wed Aug 01 00:00:00 UTC 2018

Clinicopathological description of two cases with SQSTM1 gene mutation associated with frontotemporal dementia.

Neuropathology : official journal of the Japanese Society of Neuropathology

Kovacs GG,van der Zee J,Hort J,Kristoferitsch W,Leitha T,Höftberger R,Ströbel T,Van Broeckhoven C,Matej R

Mon Feb 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43

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Gene Aliases: 1190002A23Rik; ALS10; C85084; TARDBP; TDP-43; TDP43

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UniProt ID: (Human) Q13148, (Mouse) Q921F2

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Entrez Gene ID: (Human) 23435, (Mouse) 230908, (Rat) 298648

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Function(s)

nucleotide binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA binding mRNA 3'-UTR binding protein binding identical protein binding poly(A) RNA binding nucleic acid binding DNA binding

Process(es)

negative regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mRNA processing RNA splicing negative regulation of gene expression nuclear fragmentation involved in apoptotic nuclear change negative regulation by host of viral transcription positive regulation of transcription from RNA polymerase II promoter regulation of cell cycle 3'-UTR-mediated mRNA stabilization nuclear inner membrane organization positive regulation of insulin secretion response to endoplasmic reticulum stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-TDP43 (Ser409, Ser410) Monoclonal Antibody (1A2C1)

Phospho-TDP43 (Ser409, Ser410) Antibody (66318-1-IG) in WB

Product Details

66318-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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