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Invitrogen
Phospho-TOP2A (Ser1337) Polyclonal Antibody
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FIGURE: 1 / 7
Phospho-TOP2A (Ser1337) Antibody (PA5-118788) in ICC/IF
Immunocytochemistry analysis of Phospho-TOP2A (Ser1337) in A549 cells (UV treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-TOP2A (Ser1337) polyclonal antibody (Product # PA5-118788) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (... View More
Product Details
PA5-118788
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TOP2A(Accession P11388), corresponding to amino acid residues around phosphorylated Ser1337.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2903288
Product Specific Information
Antibody detects endogenous levels of TOP2A only when phosphorylated at Ser1337.
Target Information
Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA Topoisomerase II alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; topoisomerase (DNA) 2 alpha; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha
Gene Aliases: Top-2; TOP2; TOP2A; TP2A
UniProt ID: (Human) P11388, (Mouse) Q01320
Entrez Gene ID: (Human) 7153, (Mouse) 21973, (Rat) 360243
magnesium ion binding
DNA binding
chromatin binding
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
protein binding
ATP binding
protein C-terminus binding
DNA-dependent ATPase activity
drug binding
DNA binding, bending
enzyme binding
protein homodimerization activity
histone deacetylase binding
ubiquitin binding
poly(A) RNA binding
protein heterodimerization activity
nucleotide binding
DNA topoisomerase activity
isomerase activity
sequence-specific DNA binding
structure-specific DNA binding
metal ion binding
resolution of meiotic recombination intermediates
sister chromatid segregation
hematopoietic progenitor cell differentiation
DNA topological change
DNA ligation
DNA unwinding involved in DNA replication
mitotic recombination
cellular response to DNA damage stimulus
chromosome segregation
protein sumoylation
apoptotic chromosome condensation
embryonic cleavage
regulation of circadian rhythm
positive regulation of apoptotic process
mitotic DNA integrity checkpoint
positive regulation of viral genome replication
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
DNA metabolic process
chromosome condensation
brain development
aging
cerebellar Purkinje cell differentiation
cerebellar granule cell differentiation
response to drug
response to parathyroid hormone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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