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Invitrogen
Phospho-TPH1 (Ser260) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-TPH1 (Ser260) Antibody (PA1-4645) in WB
Western blot of TPH1 in rat brainstem lysate showing specific immunolabeling of a band at ~55 kDa corresponding to Phospho-TPH1 (Ser260) polyclonal antibody (Product # PA1-4645 in lane one (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by pre-adsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (NP), shown in the third l... View More
Product Details
PA1-4645
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of rat TPH1 conjugated to KLH
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2207558
Product Specific Information
This antibody is predicted to react with bovine, canine, chicken, mouse and zebrafish based on 100% sequence homology.
This antibody is specific for the ~55 kDa Tryptophan Hydroxylase protein phosphorylated at Ser260 in Western blots of human dorsal Raphe nucleus.
Target Information
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase)
Gene Aliases: TPH; TPH1; TPRH; TRPH
UniProt ID: (Human) P17752, (Rat) P09810
Entrez Gene ID: (Human) 7166, (Rat) 24848
circadian rhythm
aromatic amino acid family metabolic process
negative regulation of ossification
response to immobilization stress
serotonin biosynthetic process
positive regulation of fat cell differentiation
indolalkylamine biosynthetic process
bone remodeling
oxidation-reduction process
mammary gland alveolus development
serotonin biosynthetic process from tryptophan
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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