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Phospho-TPL2 (Ser400) Antibody (PA5-37525) in IHC (P)
Immunohistochemical analysis of Phospho-TPL2 (Ser400) in paraffin-embedded Human brain tissue using Phospho-TPL2 (Ser400) Polyclonal Antibody (Product # PA5-37525) (left) or the same antibody preincubated with blocking peptide (right).
Product Details
PA5-37525
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence around phosphorylation site of Serine 400(C-Q-S(p)-L-D) derived from HumanMAP3K8.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2554134
Product Specific Information
A suggested positive control for IHC is human brain tissue.
Target Information
MAP3K8 (COT) is a serine/threonine kinase which can activate both the MAP and JNK kinase pathways and plays a role in the cell cycle. Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK8 is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: augmented in rheumatoid arthritis 2; Cancer Osaka thyroid oncogene; CCOT; cot (cancer Osaka thyroid) oncogene; EC 1.14.16.2; Ewing sarcoma transformant; FLJ10486; M3K8; MAP3K 8; Mitogen-activated protein kinase kinase kinase 8; Protein kinase cot; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TH isoform 3; TH isoform a; TH-4; TPL 2; TPL-2; Tumor progression locus 2; TY3H; TYH
Gene Aliases: AURA2; c-COT; COT; EST; ESTF; MAP3K8; MEKK8; Tpl-2; TPL2
UniProt ID: (Human) P41279
Entrez Gene ID: (Human) 1326
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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