Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Phospho-TRPM7 (Ser1569) Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
Phospho-TRPM7 (Ser1569) Antibody (PA5-143780) in WB
Western blot of Phospho-TRPM7 (Ser1569) in rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit anti-TRPM7 (Extracellular region) polyclonal antibody (lanes 1 & 2) or Phospho-TRPM7 (Ser1569) polyclonal antibody (Product # PA5-143780) (lanes 3 & 4).
Product Details
PA5-143780
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-TRPM7 (Ser-1569) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1569 in human TRPM7. This site is well conserved in rat and mouse TRPM7, and is conserved in TRPM6 (Thr-1724) however the site is threonine instead of serine. This site has no homology to other TRPM family members.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2943008
Product Specific Information
The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1569) then affinity purified using phospho-TRPM7 (Ser-1569) peptide. This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 stimulated with calyculin A and rat PC12 cells, as well as detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.
Target Information
TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Channel kinase 1; Channel-kinase 1; FLJ20117; Long transient receptor potential channel 7; long transient receptor potential channel 7 {ECO:0000250|UniProtKB:Q96QT4}; LTRPC ion channel family member 7; LTrpC-7; LTrpC7 {ECO:0000250|UniProtKB:Q96QT4}; tr; Transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel subfamily M member 7 {ECO:0000312|RGD:620053}; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential M7; Transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-phospholipase C-interacting kinase {ECO:0000303|PubMed:11161216}; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; TRP-PLIK; TRP-PLIK {ECO:0000303|PubMed:11161216}; trpm7 {ECO:0000312|RGD:620053}
Gene Aliases: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Ltpr7; LTrpC-7; LTRPC7; TRP-PLIK; TRPM7; TRPPLIK
UniProt ID: (Human) Q96QT4, (Mouse) Q923J1, (Rat) Q925B3
Entrez Gene ID: (Human) 54822, (Mouse) 58800, (Rat) 679906
actin binding
protein serine/threonine kinase activity
calcium channel activity
ATP binding
myosin binding
metal ion binding
nucleotide binding
protein kinase activity
ion channel activity
cation channel activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
cellular magnesium ion homeostasis
calcium-dependent cell-matrix adhesion
actomyosin structure organization
protein autophosphorylation
protein tetramerization
necroptotic process
calcium ion transmembrane transport
protein phosphorylation
transport
ion transport
cation transport
calcium ion transport
memory
programmed cell death
phosphorylation
positive regulation of apoptotic process
transmembrane transport
response to radiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support