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Phospho-TSC2 (Ser939) Antibody (710395) in ICC/IF
Immunofluorescent analysis of Phospho-Tuberin pSer939 in U2OS cells using a Phospho-Tuberin pSer939 Recombinant Rabbit Superclonal™ Antibody (Product # 710395) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (blue) (Image D) and actin stained with Alexa Fluor 594 phalloidin (red) (image B). Image C is a composite... View More
Product Details
710395
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
23HCLC
Immunogen
Phosphoeptide corresponding to amino acids 935-944 of human TSC2 [pS939]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532706
Product Specific Information
This antibody is predicted to react with chimpanzee, equine and non-human primate based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2), is implicated as a tumor suppressor and may function in vesicular transport, play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking. TSC2 specifically stimulates the intrinsic GTPase activity of the Ras related protein RAP1A and RAB5, suggesting a possible mechanism for its role in regulating cellular growth. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors and are involved in diseases such as lymphangioleiomyomatosis and TSC2 angiomyolipoma. Alternative splicing results in transcript variants of three different isoforms of TSC2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000198394; protein phosphatase 1, regulatory subunit 160; Tuberin; Tuberous sclerosis 2 protein; tuberous sclerosis 2 protein homolog
Gene Aliases: LAM; Nafld; PPP1R160; Tcs2; TSC2; TSC4
UniProt ID: (Human) P49815
Entrez Gene ID: (Human) 7249, (Mouse) 22084
neural tube closure
negative regulation of protein kinase activity
protein import into nucleus
endocytosis
cell cycle arrest
heart development
protein localization
negative regulation of cell proliferation
negative regulation of phosphatidylinositol 3-kinase signaling
viral process
vesicle-mediated transport
regulation of endocytosis
negative regulation of Wnt signaling pathway
negative regulation of TOR signaling
protein kinase B signaling
positive regulation of GTPase activity
regulation of insulin receptor signaling pathway
negative regulation of insulin receptor signaling pathway
insulin-like growth factor receptor signaling pathway
positive chemotaxis
regulation of small GTPase mediated signal transduction
regulation of cell cycle
negative regulation of protein kinase B signaling
response to hypoxia
signal transduction
phosphatidylinositol 3-kinase signaling
cell projection organization
cellular response to insulin stimulus
negative regulation of MAP kinase activity
protein transport into plasma membrane raft
establishment of protein localization
negative regulation of cell size
positive regulation of transcription from RNA polymerase II promoter
glucose import
negative regulation of epithelial cell proliferation
protein homooligomerization
protein heterooligomerization
Performance Guarantee
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