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Phospho-TUBA1C (Ser165) Antibody (PA5-143783) in WB
Western blot of Phospho-TUBA1C (Ser165) in 250 ng/lane of a1-Tubulin unphosphorylated (lanes 1, 3, & 5) or phosphorylated at Ser-165 with PKCalpha (lanes 2, 4, & 6). The blots were probed with TUBA1C polyclonal antibody (Product # PA5-143782) (lanes 1 & 2), Phospho-TUBA1C (Ser165) polyclonal antibody (Product # PA5-143783) (lanes 3 & 4), and anti-alpha-Tubulin (C-terminus) (lanes 5 & 6).
Product Details
PA5-143783
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Phospho-alpha6-Tubulin (Ser-165) synthetic peptide (coupled to KLH) corresponds to amino acid residues around serine 165 of human alpha6-Tubulin. This sequence is well conserved in most alpha-tubulin isoforms from most eukaryotic species, but is not conserved in beta-Tubulin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2943011
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated alpha6-Tubulin (a.a. 160-169) peptide before affinity purification using alpha6-Tubulin (Ser-165) peptide (without carrier). The antibody detects a 50 kDa* protein corresponding to the molecular mass of alpha-Tubulin on SDS-PAGE immunoblots of recombinant a1-tubulin phosphorylated with PKCalpha. This reactivity is not observed after lambda phosphatase treatment.
Target Information
TUBA1C (Tubulin Alpha 1c) is a Protein Coding gene. Diseases associated with TUBA1C include Distal Hereditary Motor Neuropathy, Type Ii. Among its related pathways are Development Slit-Robo signaling and Gap junction. Gene Ontology (GO) annotations related to this gene include GTP binding and structural molecule activity. An important paralog of this gene is TUBA1B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-6; Tubulin alpha-1C chain; Tubulin alpha-6 chain; tubulin, alpha 1c; tubulin, alpha 6
Gene Aliases: bcm948; M[a]6; TUBA1C; TUBA6
UniProt ID: (Human) Q9BQE3, (Rat) Q6AYZ1, (Mouse) P68373
Entrez Gene ID: (Human) 84790, (Rat) 300218, (Mouse) 22146
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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