Invitrogen

Phospho-Tyrosine Hydroxylase (Ser40) Polyclonal Antibody

Phospho-Tyrosine Hydroxylase (Ser40) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Tyrosine Hydroxylase (Ser40) Antibody (36-8600) in ICC/IF

Immunofluorescence analysis of Phospho-Tyrosine Hydroxylase (Ser40) Polyclonal Antibody was performed using 70% confluent log phase PC-12 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Phospho-Tyrosine Hydroxylase (Ser40) Polyclonal Antibody (Pro... View More

Phospho-Tyrosine Hydroxylase (Ser40) Antibody in Immunohistochemistry (IHC)

Phospho-Tyrosine Hydroxylase (Ser40) Antibody in Western Blot (WB)

Phospho-Tyrosine Hydroxylase (Ser40) Antibody

Product Image Gallery

Product Details

36-8600

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

1:1,000

View 3 publications 3 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide derived from the rat tyrosine hydroxylase (TH) protein (aa 40).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 50% glycerol, 100µg/mL BSA, 0.15M NaCl

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_138590

Target Information

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Protein tyrosine phosphatase receptor type delta (PTPRD) gene in an animal model of restless legs syndrome.

Journal of sleep research

Morais MA,Franco BS,Holanda ASS,de Paula Simino LA,Veras ACC,Torsoni MA,Manconi M,Torsoni AS,Esteves AM

36-8600 was used in Western Blot to suggest that the behavioural, physiological, and molecular changes observed in SHRs provide a useful animal model of RLS, reinforcing the importance of this strain as an animal model of this sleep disorder.

Sat Apr 01 00:00:00 UTC 2023

Partial loss of arcuate kisspeptin neurons in female rats stimulates luteinizing hormone and decreases prolactin secretion induced by estradiol.

Journal of neuroendocrinology

Campideli-Santana AC,Gusmao DO,Almeida FRCL,Araujo-Lopes R,Szawka RE

36-8600 was used in Immunohistochemistry-immunofluorescence to investigate the impact of the reduction of KNDy neurons on the estradiol (E2) regulation of gonadal and prolactin (PRL) axes.

Tue Nov 01 00:00:00 UTC 2022

Docosahexaenoic acid protects motor function and increases dopamine synthesis in a rat model of Parkinson's disease via mechanisms associated with increased protein kinase activity in the striatum.

Neuropharmacology

Chitre NM,Wood BJ,Ray A,Moniri NH,Murnane KS

36-8600 was used in Western Blotting to describe the neuroprotective effects of the omega-3 fatty acid docosahexaenoic acid (DHA) in the medial forebrain bundle 6-hydroxydopamine (6-OHDA) model of advanced-stage PD in rats.

Fri May 01 00:00:00 UTC 2020

Reduced dopaminergic tone during lactation is permissive to the hypothalamic stimulus for suckling-induced prolactin release.

Journal of neuroendocrinology

Silva KSC,Aquino NSS,Gusmao DO,Henriques PC,Reis AM,Szawka RE

36-8600 was used in Immunohistochemistry-immunofluorescence to provide evidence that dopamine synthesis and release are tonically reduced during lactation, whereas dopamine is still functional with respect to inhibiting PRL secretion.

Sun Nov 01 00:00:00 UTC 2020

Reduced dopaminergic tone during lactation is permissive to the hypothalamic stimulus for suckling-induced prolactin release.

Journal of neuroendocrinology

Silva KSC,Aquino NSS,Gusmao DO,Henriques PC,Reis AM,Szawka RE

Sun Nov 01 00:00:00 UTC 2020

Elevated Prolactin during Pregnancy Drives a Phenotypic Switch in Mouse Hypothalamic Dopaminergic Neurons.

Cell reports

Yip SH,Romanò N,Gustafson P,Hodson DJ,Williams EJ,Kokay IC,Martin AO,Mollard P,Grattan DR,Bunn SJ

36-8600 was used in Western Blotting to provide evidence of a physiologically important, reversible alteration in the behavior of a specific population of hypothalamic neurons in the adult brain via elevated prolactin levels.

Tue Feb 12 00:00:00 UTC 2019

α-Estrogen and Progesterone Receptors Modulate Kisspeptin Effects on Prolactin: Role in Estradiol-Induced Prolactin Surge in Female Rats.

Endocrinology

Aquino NSS,Araujo-Lopes R,Henriques PC,Lopes FEF,Gusmao DO,Coimbra CC,Franci CR,Reis AM,Szawka RE

36-8600 was used in Immunohistochemistry-immunofluorescence to investigate the interaction between ovarian steroid receptors and kisspeptin in the control of prolactin secretion.

Thu Jun 01 00:00:00 UTC 2017

Hypothalamic Effects of Tamoxifen on Oestrogen Regulation of Luteinising Hormone and Prolactin Secretion in Female Rats.

Journal of neuroendocrinology

Aquino NS,Araujo-Lopes R,Batista IA,Henriques PC,Poletini MO,Franci CR,Reis AM,Szawka RE

36-8600 was used in immunohistochemistry - free floating to study prolactin secretion in female rats and oestrogen regulation of luteinising hormone by the hypothalamic effects of tamoxifen

Fri Jan 01 00:00:00 UTC 2016

Litter size reduction alters insulin signaling in the ventral tegmental area and influences dopamine-related behaviors in adult rats.

Behavioural brain research

Portella AK,Silveira PP,Laureano DP,Cardoso S,Bittencourt V,Noschang C,Werlang I,Fontella FU,Dalmaz C,Goldani MZ

36-8600 was used in western blot to investigate the effect of early postnatal overfeeding on insulin signaling in the ventral tegmental area.

Sun Feb 01 00:00:00 UTC 2015

Progesterone decreases tyrosine hydroxylase phosphorylation state and increases protein phosphatase 2A activity in the stalk-median eminence on proestrous afternoon.

The Journal of endocrinology

Liu B,Arbogast LA

Mon Feb 01 00:00:00 UTC 2010

Phosphorylation state of tyrosine hydroxylase in the stalk-median eminence is decreased by progesterone in cycling female rats.

Endocrinology

Liu B,Arbogast LA

36-8600 was used in western blot to study the role of tyrosine hydroxylase in the stalk-median eminence of female rats.

Tue Apr 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

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Gene Aliases: Th; The

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UniProt ID: (Rat) P04177

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Entrez Gene ID: (Rat) 25085

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Function(s)

monooxygenase activity tyrosine 3-monooxygenase activity protein binding ferrous iron binding ferric iron binding amino acid binding oxygen binding enzyme binding protein domain specific binding tetrahydrobiopterin binding dopamine binding

Process(es)

response to hypoxia synaptic transmission, dopaminergic response to amphetamine dopamine biosynthetic process from tyrosine fatty acid metabolic process sphingolipid metabolic process heart development visual perception sensory perception of sound learning memory mating behavior locomotory behavior regulation of heart contraction response to water deprivation response to light stimulus response to herbicide response to salt stress organ morphogenesis response to metal ion response to zinc ion multicellular organism aging response to organic cyclic compound response to activity aminergic neurotransmitter loading into synaptic vesicle glycoside metabolic process response to insecticide phthalate metabolic process cerebral cortex development response to nutrient levels response to estradiol response to lipopolysaccharide isoquinoline alkaloid metabolic process response to nicotine social behavior cellular response to drug response to isolation stress response to immobilization stress neurotransmitter biosynthetic process terpene metabolic process dopamine biosynthetic process epinephrine biosynthetic process norepinephrine biosynthetic process catecholamine biosynthetic process eye photoreceptor cell development response to drug circadian sleep/wake cycle eating behavior response to peptide hormone response to ethanol response to ether response to pyrethroid response to steroid hormone embryonic camera-type eye morphogenesis cognition protein homotetramerization response to corticosterone response to electrical stimulus phytoalexin metabolic process oxidation-reduction process response to growth factor cellular response to manganese ion cellular response to alkaloid cellular response to nicotine cellular response to glucose stimulus cellular response to growth factor stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-Tyrosine Hydroxylase (Ser40) Polyclonal Antibody

Phospho-Tyrosine Hydroxylase (Ser40) Antibody (36-8600) in ICC/IF

Product Details

36-8600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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