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Invitrogen
Phospho-ULK1 (Ser757) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-ULK1 (Ser757) Antibody (PA5-121296) in WB
Western blot analysis of Phospho-ULK1 (Ser757) in extracts of A-431 cells. Samples were incubated with Phospho-ULK1 (Ser757) Polyclonal antibody (Product # PA5-121296) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. A-431 cells were treated by EGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg p... View More
Product Details
PA5-121296
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around S757 of human Phospho-ULK1-S757 (NP_0035561)
Conjugate
Form
Liquid
Concentration
0.56 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914868
Product Specific Information
Positive test controls include: A-431. The target is usually found in the following locations: Cytoplasm, Preautophagosomal structure, cytosol.
Immunogen sequence: TVGSP
Target Information
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; ULK 1; UNC 51; Unc-51-like kinase 1
Gene Aliases: ATG1; ATG1A; hATG1; KIAA0722; ULK1; UNC51; Unc51.1
UniProt ID: (Human) O75385
Entrez Gene ID: (Human) 8408
protein phosphorylation
signal transduction
protein localization
regulation of autophagy
positive regulation of autophagy
macroautophagy
positive regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection regeneration
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
response to starvation
protein autophosphorylation
axon extension
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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