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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • VE-cadherin (CD144) Antibodies

          Invitrogen

          Phospho-VE-cadherin (Tyr658) Polyclonal Antibody

          Advanced Verification
          13 Published Figures
          23 References
          View all (56) VE-cadherin (CD144) antibodies

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          Datasheet
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          Cite Phospho-VE-cadherin (Tyr658) Polyclonal Antibody

          • Antibody Testing Data (1)
          • Published Figures (13)
          • Advanced Verification (1)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

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          Phospho-VE-cadherin (Tyr658) Antibody (44-1144G) in WB

          Extracts of HUVEC cells either untreated (1) or treated (2-5)with 100 ng/mL VEGF for 10. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the VE-cadherin (pY658) (Product # 44-1144G). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody in Western Blot (WB)
          Phospho-VE-cadherin (Tyr658) Antibody

          Product Details

          44-1144G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 16 publications 16 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Hamster, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VE-cadherin that contains tyrosine 658. This sequence is conserved in chicken, chimpanzee, cow, dog, mouse, pig, and rat.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533583

          Product Specific Information

          This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated VE-cadherin. The final product is generated by affinity chromatography using an VE-cadherin-derived peptide that is phosphorylated at tyrosine 658.

          Positive controls used with this antibody in western blotting were HUVEC +/- VEGF.

          Target Information

          VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 7B4 antigen; 7B4/cadherin-5; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

          View more View less

          Gene Aliases: 7B4; AA408225; CD144; CDH5; VE-Cad; Vec; VEcad; VECD

          View more View less

          UniProt ID: (Human) P33151, (Mouse) P55284

          View more View less

          Entrez Gene ID: (Human) 1003, (Mouse) 12562

          View more View less

          Function(s)
          receptor binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding ion channel binding metal ion binding
          Process(es)
          blood vessel maturation cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules negative regulation of cell proliferation single organismal cell-cell adhesion regulation of establishment of cell polarity cell-cell junction assembly transforming growth factor beta receptor signaling pathway adherens junction organization positive regulation of establishment of endothelial barrier
          It has to be done as per old AB suggested Products section.
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