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Phospho-Vimentin (Ser56) Antibody (711212) in ICC/IF
For immunofluorescence analysis, SW480 cells were fixed and permeabilized for detection of endogenous Vimentin pS56 using Anti-Vimentin pS56 Recombinant Rabbit Superclonal™ Antibody (Product # 711212, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mou... View More
Product Details
711212
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
7HCLC
Immunogen
Peptide corresponding to Human VIM (aa 53-61)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633001
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Pig and Sheep
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin
Gene Aliases: CTRCT30; HEL113; VIM
UniProt ID: (Human) P08670, (Rat) P31000
Entrez Gene ID: (Human) 7431, (Rat) 81818
glycoprotein binding
double-stranded RNA binding
structural constituent of cytoskeleton
structural constituent of eye lens
protein binding
protein C-terminus binding
identical protein binding
scaffold protein binding
keratin filament binding
structural molecule activity
kinase binding
protein kinase binding
protein phosphatase 2A binding
movement of cell or subcellular component
positive regulation of gene expression
negative regulation of neuron projection development
astrocyte development
viral process
muscle filament sliding
intermediate filament organization
Bergmann glial cell differentiation
SMAD protein signal transduction
lens fiber cell development
aging
response to radiation
response to zinc ion
response to selenium ion
response to estradiol
response to vitamin D
skeletal muscle tissue regeneration
cellular response to fibroblast growth factor stimulus
intermediate filament-based process
fat cell differentiation
decidualization
regulation of axonogenesis
positive regulation of glial cell proliferation
regulation of Schwann cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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