Invitrogen

Phospho-alpha Actinin 1/2/3/4 (Ser159) Polyclonal Antibody

View all (3) alpha Actinin 1/2/3/4 antibodies

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-114548

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ACTN1/2/3/4 around the phosphorylation site of Ser159.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2899184

Product Specific Information

Antibody detects endogenous levels of ACTN1/2/3/4 only when phosphorylated at Ser159.

Target Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: actinin 1 smooth muscle; actinin, alpha 1; actinin, alpha 2; actinin, alpha 3; alpha actinin 1a; alpha actinin 4; Alpha-actinin cytoskeletal isoform; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-1; Alpha-actinin-2; Alpha-actinin-3; Alpha-actinin-4; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; non-muscle alpha-actinin 1; Non-muscle alpha-actinin 4; Non-muscle alpha-actinin-1

View more View less

Gene Aliases: 1110008F24Rik; 3110023F10Rik; ACTININ-4; ACTN1; Actn1a; ACTN2; ACTN3; ACTN4; BDPLT15; C77391; CMD1AA; CMH23; FSGS; FSGS1

View more View less

UniProt ID: (Human) P12814, (Human) P35609, (Human) Q08043, (Human) O43707, (Rat) Q9Z1P2, (Mouse) Q7TPR4, (Mouse) Q9JI91, (Mouse) O88990, (Mouse) P57780, (Rat) Q9QXQ0

View more View less

Entrez Gene ID: (Human) 87, (Human) 88, (Human) 89, (Human) 81, (Rat) 81634, (Mouse) 109711, (Mouse) 11472, (Rat) 291245, (Mouse) 11474, (Rat) 171009, (Mouse) 60595, (Rat) 63836

View more View less

Function(s)
double-stranded RNA binding integrin binding calcium ion binding protein binding vinculin binding ligand-dependent nuclear receptor transcription coactivator activity protein homodimerization activity ion channel binding actin filament binding Ras guanyl-nucleotide exchange factor activity phosphatidylinositol-4,5-bisphosphate binding cytoskeletal protein binding structural constituent of muscle protein domain specific binding LIM domain binding thyroid hormone receptor coactivator activity titin binding identical protein binding protein dimerization activity FATZ binding titin Z domain binding actin binding RNA polymerase II regulatory region sequence-specific DNA binding nucleoside binding chromatin DNA binding nuclear hormone receptor binding retinoic acid receptor binding poly(A) RNA binding protein N-terminus binding metal ion binding protein binding, bridging protein complex binding
Process(es)
platelet degranulation actin filament organization platelet activation platelet formation platelet morphogenesis regulation of apoptotic process focal adhesion assembly actin filament bundle assembly negative regulation of cellular component movement actin filament network formation actin crosslink formation platelet aggregation regulation of nucleic acid-templated transcription MAPK cascade cell adhesion microspike assembly muscle filament sliding regulation of membrane potential negative regulation of potassium ion transport positive regulation of potassium ion transport positive regulation of GTPase activity sarcomere organization protein homotetramerization actin filament uncapping cardiac muscle cell development phospholipase C-activating angiotensin-activated signaling pathway establishment of protein localization to plasma membrane negative regulation of potassium ion transmembrane transporter activity positive regulation of potassium ion transmembrane transporter activity negative regulation of protein localization to cell surface positive regulation of receptor activity positive regulation of endocytic recycling positive regulation of cation channel activity regulation of the force of skeletal muscle contraction skeletal muscle atrophy transition between fast and slow fiber response to denervation involved in regulation of muscle adaptation negative regulation of glycolytic process positive regulation of skeletal muscle tissue growth negative regulation of calcineurin-NFAT signaling cascade negative regulation of oxidative phosphorylation regulation of aerobic respiration positive regulation of glucose catabolic process to lactate via pyruvate cortical cytoskeleton organization muscle contraction transport protein transport peroxisome proliferator activated receptor signaling pathway retinoic acid receptor signaling pathway positive regulation of pinocytosis positive regulation of cellular component movement bicellular tight junction assembly protein localization to bicellular tight junction response to hypoxia vesicle transport along actin filament positive regulation of cell migration positive regulation of sodium:proton antiporter activity negative regulation of substrate adhesion-dependent cell spreading positive regulation of NIK/NF-kappaB signaling
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support