Invitrogen

Phospho-c-Myc (Ser62) Polyclonal Antibody

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View all (122) c-Myc antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-104729

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MYC(Accession P01106), corresponding to amino acid residues around phosphorylated Ser62.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816202

Product Specific Information

Antibody detects endogenous levels of c-Myc only when phosphorylated at Serine 62.

Target Information

The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Avian myelocytomatosis viral (v-myc) oncogene homolog; avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; c-myc epitope tag; c-myc proto-oncogene; Class E basic helix-loop-helix protein 39; cmyc; Myc protein; Myc proto-oncogene protein; myc-related translation/localization regulatory factor; myelocytomatosis viral oncogene homolog; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog

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Gene Aliases: AU016757; BHLHE39; c-Myc; mMyc; MRTL; MYC; Myc2; MYCC; Niard; Nird; RNCMYC

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UniProt ID: (Human) P01106, (Rat) P09416, (Mouse) P01108

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Entrez Gene ID: (Human) 4609, (Rat) 24577, (Mouse) 17869

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein complex binding protein dimerization activity repressing transcription factor binding E-box binding core promoter proximal region sequence-specific DNA binding double-stranded DNA binding sequence-specific DNA binding protein heterodimerization activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade branching involved in ureteric bud morphogenesis in utero embryonic development B cell apoptotic process positive regulation of mesenchymal cell proliferation regulation of oxidative phosphorylation positive regulation of B cell apoptotic process glucose metabolic process chromatin remodeling transcription, DNA-templated DNA-templated transcription, initiation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter pyruvate transport amino acid transport cellular iron ion homeostasis activation of cysteine-type endopeptidase activity involved in apoptotic process cellular response to DNA damage stimulus inner mitochondrial membrane organization cell cycle arrest regulation of mitotic cell cycle cell proliferation positive regulation of cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage glycoprotein metabolic process response to radiation pathogenesis response to wounding flavonoid metabolic process response to gamma radiation regulation of gene expression negative regulation of gene expression positive regulation of mitochondrial membrane potential Wnt signaling pathway protein processing transformation of host cell by virus hypothalamus development negative regulation of protein binding regulation of telomere maintenance response to estradiol cellular response to UV cellular response to drug skeletal muscle cell differentiation middle ear morphogenesis response to drug regulation of apoptotic process positive regulation of catalytic activity response to alkaloid positive regulation of cysteine-type endopeptidase activity involved in apoptotic process pigmentation canonical Wnt signaling pathway involved in positive regulation of wound healing canonical Wnt signaling pathway involved in negative regulation of apoptotic process canonical Wnt signaling pathway involved in positive regulation of apoptotic process G0 to G1 transition negative regulation of monocyte differentiation positive regulation of cell cycle positive regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import lactic acid secretion positive regulation of fibroblast proliferation negative regulation of fibroblast proliferation skeletal system morphogenesis positive regulation of epithelial cell proliferation detection of mechanical stimulus involved in sensory perception of sound chromosome organization negative regulation of cell division canonical Wnt signaling pathway negative regulation of cell death cellular response to carbohydrate stimulus cellular response to interleukin-1 cellular response to estrogen stimulus cellular response to organic cyclic compound cellular response to hydrostatic pressure fungal-type cell wall polysaccharide metabolic process positive regulation of metanephric cap mesenchymal cell proliferation positive regulation of cellular respiration positive regulation of DNA biosynthetic process positive regulation of response to DNA damage stimulus positive regulation of ATP biosynthetic process positive regulation of apoptotic signaling pathway energy reserve metabolic process Notch signaling pathway oxygen transport negative regulation of stress-activated MAPK cascade negative regulation of apoptotic process fibroblast apoptotic process response to growth factor beta-catenin-TCF complex assembly
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