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          • Primary Antibodies ›
          • eNOS Antibodies

          Invitrogen

          Phospho-eNOS (Ser1179) Polyclonal Antibody

          2 Published Figures
          7 References
          View all (45) eNOS antibodies

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          Cite Phospho-eNOS (Ser1179) Polyclonal Antibody

          • Antibody Testing Data (1)
          • Published Figures (2)
          Phospho-eNOS (Ser1179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Phospho-eNOS (Ser1179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Phospho-eNOS (Ser1179) Antibody (36-9100) in IHC (P)

          Immunohistochemistry analysis of Phospho-eNOS (pSer1179) showing staining in the membrane of paraffin-embedded human placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at r... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-eNOS (Ser1179) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Phospho-eNOS (Ser1179) Antibody in Western Blot (WB)
          Phospho-eNOS (Ser1179) Antibody in Western Blot (WB)

          Product Details

          36-9100

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 5 publications 5 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20
          -

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          Assay-dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bovine

          Published species

          Bovine, Human, Mouse, Non-human primate, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide derived from the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.01% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533285

          Target Information

          Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cNOS; constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial NOS; eNOS; nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

          View more View less

          Gene Aliases: BOS_4740

          View more View less

          Entrez Gene ID: (Bovine) 287024

          View more View less

          Function(s)
          nitric-oxide synthase activity iron ion binding calmodulin binding FMN binding heme binding flavin adenine dinucleotide binding NADP binding
          Process(es)
          arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
          It has to be done as per old AB suggested Products section.
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