Invitrogen

Phospho-eNOS (Thr495) Polyclonal Antibody

3 Published Figures
4 References
View all (45) eNOS antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-17706

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 4 publications 4 publications

Immunoprecipitation (IP)

1:100
-
Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr495 of human eNOS
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

44 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982870

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

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Gene Aliases: BOS_4740; cNOS; EC-NOS; ECNOS; eNOS; NOS; NOS3; NOSIII

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UniProt ID: (Pig) Q28969, (Human) P29474

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Entrez Gene ID: (Bovine) 287024, (Pig) 397557, (Human) 4846

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Function(s)
nitric-oxide synthase activity iron ion binding calmodulin binding FMN binding heme binding flavin adenine dinucleotide binding NADP binding actin monomer binding protein binding tetrahydrobiopterin binding arginine binding cadmium ion binding scaffold protein binding
Process(es)
arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity negative regulation of extrinsic apoptotic signaling pathway via death domain receptors angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin nitric oxide mediated signal transduction regulation of blood pressure negative regulation of cell proliferation response to heat negative regulation of platelet activation negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development lipopolysaccharide-mediated signaling pathway response to fluid shear stress negative regulation of potassium ion transport endothelial cell migration cell redox homeostasis positive regulation of angiogenesis negative regulation of blood pressure positive regulation of vasodilation regulation of blood vessel size regulation of nitric-oxide synthase activity negative regulation of hydrolase activity negative regulation of calcium ion transport oxidation-reduction process
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