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Phospho-p130Cas (Tyr165) Antibody (PA5-78293) in ICC/IF
Phospho-p130Cas (Tyr165) Polyclonal Antibody detects p130Cas (phospho Tyr165) protein at cell junction by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: p130Cas (phospho Tyr165) protein stained by Phospho-p130Cas (Tyr165) Polyclonal Antibody (Product # PA5-78293) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-78293
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr165 of human p130Cas. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.08 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2736396
Product Specific Information
Positive Control: Neuro2A, C8D30, NIH-3T3, C2C12, Rat2
Predicted Reactivity: Mouse (100%), Rat (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCA1; breast cancer anti-estrogen resistance 1; Breast cancer anti-estrogen resistance protein 1; Cas scaffolding protein family member 1; CRK-associated substrate; Crk-associated substrate p130Cas; OTTHUMP00000174941; p130 Cas; p130cas; p130Cas (pTyr410); p130Cas (pY410); p130Cas phospho; phospho p130Cas; v-crk-associated tyrosine kinase substrate
Gene Aliases: AI385681; BCAR1; CAS; CAS1; CASS1; CRKAS; P130CAS
UniProt ID: (Human) P56945, (Mouse) Q61140, (Rat) Q63767
Entrez Gene ID: (Human) 9564, (Mouse) 12927, (Rat) 25414
actin filament organization
cell adhesion
epidermal growth factor receptor signaling pathway
G-protein coupled receptor signaling pathway
integrin-mediated signaling pathway
insulin receptor signaling pathway
positive regulation of endothelial cell migration
cell migration
actin cytoskeleton organization
positive regulation of cell migration
cellular response to hepatocyte growth factor stimulus
platelet-derived growth factor receptor signaling pathway
vascular endothelial growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
hepatocyte growth factor receptor signaling pathway
antigen receptor-mediated signaling pathway
B cell receptor signaling pathway
cell chemotaxis
signal transduction
regulation of cell growth
cell proliferation
regulation of apoptotic process
T cell receptor signaling pathway
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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