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Invitrogen
Phospho-p47phox (Ser304) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-p47phox (Ser304) Antibody (PA5-105496) in ICC/IF
Immunocytochemistry analysis of Phospho-p47phox (Ser304) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-p47phox (Ser304) polyclonal antibody (Product # PA5-105496) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated... View More
Product Details
PA5-105496
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NCF1(Accession P14598), corresponding to amino acid residues around phosphorylated Ser304.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2816924
Product Specific Information
Antibody detects endogenous levels of NCF1/p47-phox only when phosphorylated at Ser304.
Target Information
p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF-1; NCF-47K; neutrop; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; P47 phox; p47-phox; phagocyte oxidase 47 kDa; SH3 and PX domain-containing protein 1A
Gene Aliases: Ncf-1; NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
UniProt ID: (Human) P14598
Entrez Gene ID: (Human) 653361, (Rat) 114553
response to yeast
leukocyte mediated cytotoxicity
respiratory burst involved in defense response
protein targeting to membrane
leukotriene metabolic process
apoptotic process
inflammatory response
cellular defense response
cell proliferation
response to bacterium
superoxide anion generation
defense response to bacterium
respiratory burst
negative regulation of smooth muscle contraction
hydrogen peroxide biosynthetic process
defense response to Gram-positive bacterium
defense response to fungus
oxidation-reduction process
neutrophil mediated killing of gram-positive bacterium
neutrophil mediated killing of fungus
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
superoxide metabolic process
positive regulation of catalytic activity
innate immune response
cell redox homeostasis
vascular endothelial growth factor receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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