Invitrogen

Phospho-p53 (Ser15) Monoclonal Antibody (C.381.0)

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1 Published Figure
2 References
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15229

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
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Immunocytochemistry (ICC/IF)

1:100
View 1 publication 1 publication

Flow Cytometry (Flow)

1:1,600
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C.381.0

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer15 of human p53
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

765 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10980596

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with p53 phosphorylated at other sites.

Target Information

The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant tumor protein 53; OTTMUSP00000006194; p53 tumor suppressor; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

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Gene Aliases: BCC7; LFS1; P53; TP53; TRP53

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UniProt ID: (Human) P04637

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Entrez Gene ID: (Human) 7157

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding protein binding ATP binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter DNA strand renaturation base-excision repair nucleotide-excision repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator ER overload response cell cycle arrest Ras protein signal transduction multicellular organism development cell aging protein localization cell proliferation negative regulation of cell proliferation determination of adult lifespan response to X-ray response to gamma radiation positive regulation of gene expression viral process protein sumoylation cell differentiation negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator positive regulation of histone deacetylation chromatin assembly mitotic G1 DNA damage checkpoint positive regulation of protein oligomerization cellular response to UV cellular response to drug cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process entrainment of circadian clock by photoperiod proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to antibiotic positive regulation of protein export from nucleus regulation of mitochondrial membrane permeability negative regulation of fibroblast proliferation circadian behavior positive regulation of peptidyl-tyrosine phosphorylation negative regulation of helicase activity protein tetramerization negative regulation of telomerase activity positive regulation of thymocyte apoptotic process positive regulation of cell cycle arrest cellular response to hypoxia cellular response to ionizing radiation intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of release of cytochrome c from mitochondria replicative senescence oxidative stress-induced premature senescence intrinsic apoptotic signaling pathway oligodendrocyte apoptotic process positive regulation of execution phase of apoptosis positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of signal transduction by p53 class mediator regulation of cell cycle G2/M phase transition positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of reactive oxygen species metabolic process positive regulation of intrinsic apoptotic signaling pathway
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