Invitrogen

Phospho-p70 S6 Kinase (Thr389) Recombinant Rabbit Monoclonal Antibody (B2H9L2)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

701064

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

B2H9L2

Immunogen

Phosphopeptide corresponding to amino acids 384-394 of human p70 ribosomal protein S6 kinase
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532366

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 70 kDa ribosomal protein S6 kinase 1; EC 2.7.11.1; kinase p70S6K; KS6B1; p70 p70-S6K; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 kinase, alpha; p70S6K-alpha; P70S6K1; p80-S6K 1; p85 p85S6K; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A

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Gene Aliases: p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; RPS6KB1; S6K; S6K-beta-1; S6K1; STK14A

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UniProt ID: (Human) P23443

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Entrez Gene ID: (Human) 6198

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Function(s)
protein kinase activity ribosomal protein S6 kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity PDZ domain binding peptide binding identical protein binding protein phosphatase 2A binding
Process(es)
G1/S transition of mitotic cell cycle behavioral fear response skeletal muscle contraction protein phosphorylation apoptotic process signal transduction germ cell development aging response to nutrient long-term memory response to heat response to wounding response to mechanical stimulus response to toxic substance response to glucose skeletal muscle atrophy response to electrical stimulus involved in regulation of muscle adaptation positive regulation of smooth muscle cell migration cell migration TOR signaling response to lipopolysaccharide response to insulin cellular response to hormone stimulus response to testosterone response to glucagon response to tumor necrosis factor response to drug negative regulation of apoptotic process response to leucine protein kinase B signaling response to ethanol positive regulation of translation positive regulation of mitotic cell cycle positive regulation of translational initiation regulation of glucose import negative regulation of insulin receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of skeletal muscle tissue growth positive regulation of smooth muscle cell proliferation response to glucocorticoid cellular response to growth factor stimulus cellular response to organic cyclic compound negative regulation of extrinsic apoptotic signaling pathway
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