Invitrogen

Phospho-p70 S6 Kinase (Thr389) Monoclonal Antibody (R.566.2)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15202

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
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Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

R.566.2

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pThr389 of human p70 S6 kinase
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

719 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978757

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 70 kDa ribosomal protein S6 kinase 1; CG10539-PA; CG10539-PB; dS6 kinase; EC 2.7.11.1; kinase p70S6K; KS6B1; p70 p70-S6K; p70 ribosomal S6 kinase; p70 ribosomal S6 kinase alpha; p70 S6 kinase; p70 S6 kinase 1; p70 S6 kinase alpha; p70 S6 kinase, alpha; p70 S6K-alpha; p70 S6KA; p70(S6K)-alpha; p70-S6K 1; p70S6 kinase; P70S6K; p70S6K kinase; p70S6K-alpha; P70S6K1; p80-S6K 1; p85 p85S6K; protein kinase-like 64F; ribosomal protein S6 kinase; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; ribosomal S6 protein kinase; RPS6-p70-protein kinase; S6 kinase; S6-kinase; S6K; S6K kinase; S6K-beta-1; S6k-PA; S6k-PB; S6K1; S6kinase; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A

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Gene Aliases: 10539; 2610318I15Rik; 4732464A07Rik; 7-10; 7084; 70kDa; AA959758; AI256796; AI314060; CG10539; Dmel\CG10539; Dmel_CG10539; Dp70[s6k]; Dp70S6k; dps6k; DS6K; fs(3)07084; l(3)07084; p-S6K; p70 S6K; p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; p70/85s6k; p70/S6K; p70[S6 kinase]; p70[S6K]; p70[S6kinase]; p70S6K; Pk64F; Pk?6; PS6K; RPS6KB1; S6K; S6K-beta-1; S6K1; s6k11; S6k|S6K; STK14A

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UniProt ID: (Human) P23443, (Mouse) Q8BSK8, (Rat) P67999

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Entrez Gene ID: (Human) 6198, (Mouse) 72508, (Rat) 83840, (Fruit fly) 38654

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Function(s)
protein kinase activity ribosomal protein S6 kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding kinase activity PDZ domain binding peptide binding identical protein binding protein phosphatase 2A binding nucleotide binding protein serine/threonine kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
Process(es)
G1/S transition of mitotic cell cycle behavioral fear response regulation of translation protein phosphorylation apoptotic process cell cycle signal transduction germ cell development positive regulation of smooth muscle cell migration phosphorylation TOR signaling response to insulin cellular response to insulin stimulus response to tumor necrosis factor negative regulation of apoptotic process protein kinase B signaling positive regulation of translation positive regulation of mitotic cell cycle positive regulation of translational initiation regulation of glucose import negative regulation of insulin receptor signaling pathway positive regulation of skeletal muscle tissue growth positive regulation of smooth muscle cell proliferation cellular response to growth factor stimulus cellular response to organic cyclic compound negative regulation of extrinsic apoptotic signaling pathway skeletal muscle contraction aging response to nutrient long-term memory response to heat response to wounding response to mechanical stimulus response to toxic substance response to glucose skeletal muscle atrophy response to electrical stimulus involved in regulation of muscle adaptation cell migration response to lipopolysaccharide cellular response to hormone stimulus response to testosterone response to glucagon response to drug response to leucine response to ethanol phosphatidylinositol-mediated signaling response to glucocorticoid response to organic substance response to organonitrogen compound response to organic cyclic compound response to amino acid response to peptide hormone lipid metabolic process phagocytosis cellular response to DNA damage stimulus response to oxidative stress actin filament organization axon guidance myoblast fusion somatic muscle development determination of adult lifespan regulation of cell size positive regulation of autophagy positive regulation of macroautophagy positive regulation of cell growth larval feeding behavior multicellular organism growth growth positive regulation of multicellular organism growth positive regulation of cell size positive regulation of growth positive regulation of organ growth oogenesis dendrite morphogenesis synaptic growth at neuromuscular junction regulation of terminal button organization regulation of response to drug
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