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Rockland
Phospho-p90 RSK1 (Ser732) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-p90 RSK1 (Ser732) Antibody (600-401-B30) in WB
Western blot using Rocklands affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membr... View More
Product Details
600-401-B30
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Horse,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human p90 RSK1 protein phosphorylated at S732. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Partially cross reactivity may occur against opossum and chicken based on >90% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.
Target Information
RPS6KA1 (RSK1) is a growth-factor regulated serine/threonine kinase that is involved in the MAPK cascade. It is thought to control cellular proliferation and differentiation through the phosphorylation of transcription factors. RPS6KA1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPK1A; p90-RSK 1; p90RSK1; p90S6K; pp90RSK1; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RSK-1; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha-1
Gene Aliases: BOS_2367; HU-1; Mapkapk-1a; MAPKAPK1A; p90-Rsk1; p90Rsk; p90Rsk1; p90S6K; RPS6KA1; RSK; Rsk-1; RSK1; S6K-alpha-1
UniProt ID: (Human) Q15418, (Rat) Q63531, (Mouse) P18653
Entrez Gene ID: (Bovine) 533908, (Human) 6195, (Dog) 487353, (Rat) 81771, (Mouse) 20111
magnesium ion binding
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
ribosomal protein S6 kinase activity
transcription factor binding
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein phosphorylation
apoptotic process
cell cycle
signal transduction
positive regulation of cell growth
intracellular signal transduction
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of translation in response to stress
regulation of DNA-templated transcription in response to stress
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
hepatocyte proliferation
positive regulation of hepatic stellate cell activation
positive regulation of transcription, DNA-templated
regulation of translation
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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