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Alomone Labs, LTD
Piezo1 Polyclonal Antibody
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FIGURE: 1 / 2
Piezo1 Antibody (APC-087-200UL) in IHC
Expression of Piezo1 in rat olfactory bulb - Immunohistochemical staining of rat olfactory bulb sections using Anti-Piezo1 Antibody (#APC-087). A. Piezo1 staining (green) appears in the neuropil with varying intensity with in and between glomeruli. B. Nuclear staining (blue) using DAPI as the counterstain. C. Merged image of panels A and B.
Product Details
APC-087-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)EDLKPQHRRHISIR, corresponding to amino acid residues 1863-1876 of rat Piezo1, The peptide is located in an internal region of the rat Piezo1 (the exact topology of Piezo1 remains to be defined).
Conjugate
Form
Lyophilized
Concentration
0.85 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Piezo1 is a pore-forming subunit of a mechanosensitive non-specific cation channel, and generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Structurally, Piezo1 contains 36 transmembrane domains and functions as a homotetramer. Piezo1 plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, Piezo1 may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Piezo1 acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation and plays a key role in blood vessel formation and vascular structure in both development and adult physiology. The protein encoded by the Piezo1 gene is a mechanically-activated ion channel that links mechanical forces to biological signals. Defects in this gene have been associated with dehydrated hereditary stomatocytosis. Diseases associated with PIEZO1 include Dehydrated Hereditary Stomatocytosis with/without Pseudohyperkalemia And/Or Perinatal Edema and Lymphedema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: family with sequence similarity 38, member A; Membrane protein induced by beta-amyloid treatment; Mib; Piezo-type mechanosensitive ion channel component 1; Protein FAM38A; protein PIEZO1
Gene Aliases: 9630020g22; Fam38a; Mib; mKIAA0233; Piezo1
UniProt ID: (Rat) Q0KL00
Entrez Gene ID: (Rat) 361430, (Mouse) 234839
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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