Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Progen
Plakophilin 2 Monoclonal Antibody (Pkp2-518)
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Details
651167
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Pkp2-518
Immunogen
Synthetic Carboxyterminal Peptide (Aa 527 - 872) Of Human Plakophilin 2
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Specificity: plakophilin 2 in both locations: in the desmosomal plaque and in the karyoplasm. Plakophilin 2 is a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, in Merkel cells, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the nuclei (karyoplasm). Polypeptide reacting: Plakophilin 2, MW ~100 kD (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38)Positive staining was obtained with several carcinomas derived from simple-type epithelia (e.g. hepatomas, colon carcinomas) and some adenocarcinomas derived from stratified epithelia (oesophagus carcinoma).Recommendation for immunohistochemistry on frozen sections: For better accessibility of the desmosome-associated antigen frozen sections might be pre-incubated for 5 min in PBS plus 0.2% Triton X-100 prior to antibody application. Longer incubation could result in a loss of Pkp2-nuclear staining. To prevent wash-out of soluble forms of the antigen, fixation with 2% formaldehyde is recommended.
Target Information
Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 1 mediates increases in desmosomal protein content, desmosome assembly and regulation of cell migration. Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin 4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Plakophilin-2
Gene Aliases: 1200008D14Rik; 1200012P04Rik; AA516617; ARVD9; PKP2; Pkp2l
UniProt ID: (Human) Q99959, (Mouse) Q9CQ73, (Rat) F1M7L9
Entrez Gene ID: (Human) 5318, (Mouse) 67451, (Rat) 287925
molecular_function
protein kinase C binding
protein binding
sodium channel regulator activity
intermediate filament binding
protein complex scaffold
ion channel binding
alpha-catenin binding
cell adhesive protein binding involved in bundle of His cell-Purkinje myocyte communication
cadherin binding involved in cell-cell adhesion
desmosome assembly
heart development
negative regulation of cell proliferation
gap junction assembly
single organismal cell-cell adhesion
negative regulation of cell migration
adherens junction maintenance
intermediate filament bundle assembly
maintenance of organ identity
ventricular cardiac muscle tissue morphogenesis
lipid homeostasis
cardiac muscle cell action potential
cardiac muscle cell action potential involved in contraction
ventricular cardiac muscle cell action potential
cell-cell signaling involved in cardiac conduction
bundle of His cell-Purkinje myocyte adhesion involved in cell communication
regulation of heart rate by cardiac conduction
establishment of protein localization to plasma membrane
regulation of ventricular cardiac muscle cell action potential
regulation of bicellular tight junction assembly
positive regulation of sodium ion transport
cell communication by electrical coupling involved in cardiac conduction
bundle of His cell to Purkinje myocyte communication
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support