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Invitrogen
Plectin Recombinant Rabbit Monoclonal Antibody (SY29-04)
FIGURE: 1 / 6
Plectin Antibody (MA5-32102) in ICC/IF
Immunofluorescence of Plectin was performed using parental and Plectin CRISPR KO cells that were transfected with a GFP or mCherry plasmid, respectively. At 48 hrs post transfection parental and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA for 15 min and permeabilized with 0.1% Triton X-100. Cells were stained with Plectin recombinant monoclonal antibody (Product # MA5-32102) at a 1:1,000 dilution overnight at 4 deg. Secondary antibody incubation was performed ... View More
Product Details
MA5-32102
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SY29-04
Immunogen
Recombinant protein within Human Plectin aa 2-101
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809395
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes. Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans. The expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development and it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms which carry out distinct and specific functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HD1; Hemidesmosomal protein 1; PCN; Plectin; plectin 1; plectin 1, intermediate filament binding protein 500kDa; Plectin-1; Plectin-6
Gene Aliases: AA591047; AU042537; EBS1; EBSMD; EBSND; EBSO; EBSOG; EBSPA; HD1; LGMD2Q; PCN; PLEC; PLEC1; PLEC1b; PLTN
UniProt ID: (Human) Q15149, (Mouse) Q9QXS1
Entrez Gene ID: (Human) 5339, (Rat) 64204, (Mouse) 18810
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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