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Invitrogen
Plexin-B2 Monoclonal Antibody (eBio3E7 (3E7)), eBioscience™
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FIGURE: 1 / 5
Plexin-B2 Antibody (14-5665-82) in Flow
Staining of the PDV cell line with 0.25 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg of Anti-Mouse Plexin-B2 Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
Product Details
14-5665-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
eBio3E7 (3E7)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_763626
Product Specific Information
Description: Plexin-B2 is a member of the Semaphorin receptor family. The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues. Plexin-B1 is a transmembrane protein that can be cleaved coverting the single chain presursor into a heterodimeric receptor. It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos. Sema4D also plays a role in the immune system interacting with CD72. Plexin-B2's possible role in the immune system has not been characterized.
Applications Reported: This eBio3E7 (3E7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.
Applications Tested: This eBio3E7 (3E7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Plexin-B2 is a member of the Semaphorin receptor family. The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues. Plexin-B1 is a transmembrane protein that can be cleaved converting the single chain precursor into a heterodimeric receptor. It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos. Sema4D also plays a role in the immune system interacting with CD72. Plexin-B2's possible role in the immune system has not been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: differentially expressed in brain tumors; MM1KIAA0315dJ402G11.3; Plexin-B2
Gene Aliases: Debt; plexin-B2; Plxnb2
UniProt ID: (Mouse) B2RXS4
Entrez Gene ID: (Mouse) 140570
neural tube closure
regulation of protein phosphorylation
signal transduction
multicellular organism development
neuroblast proliferation
brain development
regulation of cell shape
regulation of GTPase activity
positive regulation of axonogenesis
semaphorin-plexin signaling pathway
regulation of neuron migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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