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Invitrogen
Podocalyxin Monoclonal Antibody (B34D1.3), PE, eBioscience™
Product Details
12-8873-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
B34D1.3
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10734225
Product Specific Information
Description: The monoclonal antibody B34D1.3 recognizes human podocalyxin, a protein expressed on undifferentiated human embryonic stem cells (ES), embryonic carcinoma cells (EC), and embryonic germ cells (EG). Podocalxyin is a heavily glycosylated cell adhesion protein with homology to CD34 and endoglycan. It was first identified on kidney podocytes, and then found to be upregulated in a variety of human cancers such as breast and prostate cancer. Like other stem cell-specific markers, the epitope recognized by the B34D1.3 antibody is lost upon cell differentiation.
The B34D1.3 antibody recognizes the ectodomain of podocalyxin, which is a different epitope from the Tra-1-60 and Tra-1-81 monoclonal antibodies. While Tra1-60 has been found to react with a sialidase-sensitive epitope, the epitope recognized by Tra1-81 has not been fully defined.
Applications Reported: This B34D1.3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B34D1.3 antibody has been pre-titrated and tested by flow cytometric analysis of the NTERA cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: GCTM-2 antigen; Gp200; MGC138240; PC; PCLP antibody; PCLP1 antibody; PODLX2; Podocalyxin; podocalyxin-like; Podocalyxin-like protein 1; podolyxin
Gene Aliases: Gp200; PC; PCLP; PCLP-1; PCLP1; PODXL
UniProt ID: (Human) O00592
Entrez Gene ID: (Human) 5420
cell adhesion
negative regulation of cell adhesion
cell migration
negative regulation of cell-cell adhesion
positive regulation of cell migration
regulation of microvillus assembly
positive regulation of cell-cell adhesion mediated by integrin
leukocyte migration
glomerular visceral epithelial cell development
epithelial tube formation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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