Invitrogen

Podoplanin Monoclonal Antibody (NZ-1.3), PE, eBioscience™

7 Published Figures

13 References

View all (70) Podoplanin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

Podoplanin Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 8

Podoplanin Antibody (12-9381-42) in Flow

Staining of the U87 cell line with Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or Anti-Human Podoplanin PE (purple histogram). Total viable cells were used for analysis.

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Product Details

12-9381-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 11 publications 11 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NZ-1.3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1582262

Product Specific Information

Description: Podoplanin is a 40-kDa type I transmembrane sialomucin-like glycoprotein involved in platelet aggregation and cancer metastasis. This protein is expressed on lymphatic endothelial cells and follicular dendritic cells, as well as on many human cancers, including squamous cell carcinoma, glioblastoma, seminoma, and mesothelioma. Podoplanin expression has been demonstrated to be regulated by the Fos protein of the Activating Protein-1 (AP-1) transcription factor complex. Much of its functionality arises via interaction with its endogenous receptor, C-type lectin-like receptor-2 (CLEC-2).

This antibody has been reported to block the interaction between Podoplanin and CLEC-2.

Applications Reported: This NZ-1.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This NZ-1.3 antibody has been pre-titrated and tested by flow cytometric analysis of U87 cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

PI16⁺ reticular cells in human palatine tonsils govern T cell activity in distinct subepithelial niches.

Nature immunology

De Martin A,Stanossek Y,Lütge M,Cadosch N,Onder L,Cheng HW,Brandstadter JD,Maillard I,Stoeckli SJ,Pikor NB,Ludewig B

12-9381-42 was used in Flow Cytometry to show that human tonsillar FRCs undergo dynamic reprogramming during life and respond vigorously to inflammatory perturbation in comparison to other stromal cell types.

Sat Jul 01 00:00:00 UTC 2023

Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension.

iScience

Al Hosni R,Bozec L,Roberts SJ,Cheema U

Published figure using Podoplanin monoclonal antibody (Product # 12-9381-42) in Flow Cytometry

Fri Apr 15 00:00:00 UTC 2022

Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses.

Cell research

He J,Yan J,Wang J,Zhao L,Xin Q,Zeng Y,Sun Y,Zhang H,Bai Z,Li Z,Ni Y,Gong Y,Li Y,He H,Bian Z,Lan Y,Ma C,Bian L,Zhu H,Liu B,Yue R

Published figure using Podoplanin monoclonal antibody (Product # 12-9381-42) in Flow Cytometry

Thu Jul 01 00:00:00 UTC 2021

Kaposi's sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis.

PLoS pathogens

Chen W,Ding Y,Liu D,Lu Z,Wang Y,Yuan Y

Published figure using Podoplanin monoclonal antibody (Product # 12-9381-42) in Flow Cytometry

Wed Dec 01 00:00:00 UTC 2021

Directed induction of alveolar type I cells derived from pluripotent stem cells via Wnt signaling inhibition.

Stem cells (Dayton, Ohio)

Kanagaki S,Ikeo S,Suezawa T,Yamamoto Y,Seki M,Hirai T,Hagiwara M,Suzuki Y,Gotoh S

Published figure using Podoplanin monoclonal antibody (Product # 12-9381-42) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2021

Phenotypic and functional characterization of synovial fluid-derived fibroblast-like synoviocytes in rheumatoid arthritis.

Scientific reports

Køster D,Egedal JH,Lomholt S,Hvid M,Jakobsen MR,Müller-Ladner U,Eibel H,Deleuran B,Kragstrup TW,Neumann E,Nielsen MA

12-9381-42 was used in Flow Cytometry to phenotypically and functionally characterize cultured synovial fluid-derived fibroblast-like synoviocytes.

Fri Nov 12 00:00:00 UTC 2021

Phenotypic and functional characterization of synovial fluid-derived fibroblast-like synoviocytes in rheumatoid arthritis.

Scientific reports

Køster D,Egedal JH,Lomholt S,Hvid M,Jakobsen MR,Müller-Ladner U,Eibel H,Deleuran B,Kragstrup TW,Neumann E,Nielsen MA

12-9381-42 was used in Flow Cytometry to phenotypically and functionally characterize cultured synovial fluid-derived fibroblast-like synoviocytes.

Fri Nov 12 00:00:00 UTC 2021

Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer.

International journal of molecular sciences

Wilkus K,Brodaczewska K,Kajdasz A,Kieda C

Published figure using Podoplanin monoclonal antibody (Product # 12-9381-42) in Flow Cytometry

Tue Aug 17 00:00:00 UTC 2021

Origin-Specific Adhesive Interactions of Mesenchymal Stem Cells with Platelets Influence Their Behavior After Infusion.

Stem cells (Dayton, Ohio)

Sheriff L,Alanazi A,Ward LSC,Ward C,Munir H,Rayes J,Alassiri M,Watson SP,Newsome PN,Rainger GE,Kalia N,Frampton J,McGettrick HM,Nash GB

12-9381 was used in Flow cytometry/Cell sorting to study the adhesive behaviour of mesenchymal stem cells in blood.

Sun Jul 01 00:00:00 UTC 2018

Human Extravillous Trophoblasts Penetrate Decidual Veins and Lymphatics before Remodeling Spiral Arteries during Early Pregnancy.

PloS one

He N,van Iperen L,de Jong D,Szuhai K,Helmerhorst FM,van der Westerlaken LA,Chuva de Sousa Lopes SM

12938142 was used in immunohistochemistry - paraffin section to study the invasion of maternal endometrium by extravillous trophoblasts.

Thu Aug 03 00:00:00 UTC 2017

Role of podoplanin in the high interleukin-17A secretion resulting from interactions between activated lymphocytes and psoriatic skin-derived mesenchymal cells.

Clinical and experimental immunology

Noack M,Ndongo-Thiam N,Miossec P

12-9381-42 was used in Flow cytometry/Cell sorting to study the interactions of skin-derived fibroblasts with peripheral blood mononuclear cells (PBMC) with a focus on the Th17 pathway and to identify a mechanism which leads to a high interleukin (IL)-17 secretion.

Sat Oct 01 00:00:00 UTC 2016

Rheumatoid synovial fibroblasts differentiate into distinct subsets in the presence of cytokines and cartilage.

Arthritis research & therapy

Croft AP,Naylor AJ,Marshall JL,Hardie DL,Zimmermann B,Turner J,Desanti G,Adams H,Yemm AI,Müller-Ladner U,Dayer JM,Neumann E,Filer A,Buckley CD

12-9381 was used in Flow cytometry/Cell sorting to propose that podoplanin (PDPN)-expressing cells may be an attractive therapeutic target in rheumatoid arthritis.

Fri Nov 18 00:00:00 UTC 2016

Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.

Arthritis research & therapy

Noack M,Ndongo-Thiam N,Miossec P

12-9381 was used in Flow cytometry/Cell sorting to study interactions between synoviocytes from rheumatoid arthritis patients and peripheral blood mononuclear cells, and their effect on the Th17 pathway during chronic inflammation.

Thu Jun 23 00:00:00 UTC 2016

Syk and Src family kinases regulate C-type lectin receptor 2 (CLEC-2)-mediated clustering of podoplanin and platelet adhesion to lymphatic endothelial cells.

The Journal of biological chemistry

Pollitt AY,Poulter NS,Gitz E,Navarro-Nuñez L,Wang YJ,Hughes CE,Thomas SG,Nieswandt B,Douglas MR,Owen DM,Jackson DG,Dustin ML,Watson SP

12-9381 was used in Immuno-assay to show that CLEC-2 signaling via Src family and Syk tyrosine kinases promotes platelet adhesion to lymphatic endothelial cells at low shear.

Fri Dec 26 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Aggrus; CTA-520D8.1; Glycoprotein 36; glycoprotein, 36-KD; Gp36; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; PDPN; Podoplanin; RP23-348F1.2; T1-alpha; T1A

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Gene Aliases: AGGRUS; GP36; Gp38; GP40; HT1A-1; OTS8; PA2.26; PDPN; PSEC0003; PSEC0025; T1A; T1A-2; T1A2; TI1A

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UniProt ID: (Human) Q86YL7

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Entrez Gene ID: (Human) 10630

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Function(s)

water transmembrane transporter activity folic acid transporter activity amino acid transmembrane transporter activity water channel activity

Process(es)

cell morphogenesis lymphangiogenesis prostaglandin metabolic process water transport amino acid transport signal transduction cell proliferation regulation of cell shape folic acid transport single organismal cell-cell adhesion platelet activation lung development lung alveolus development cell motility positive regulation of cellular component movement regulation of G1/S transition of mitotic cell cycle

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Podoplanin Monoclonal Antibody (NZ-1.3), PE, eBioscience™

Podoplanin Antibody (12-9381-42) in Flow

Product Details

12-9381-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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