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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Podoplanin Antibodies

          Invitrogen

          Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eBioscience™

          Advanced Verification
          29 Published Figures
          58 References
          View all (70) Podoplanin antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eBioscience™

          • Antibody Testing Data (6)
          • Published Figures (29)
          • Advanced Verification (3)
          Podoplanin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Podoplanin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 38

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          Podoplanin Antibody (14-5381-82) in IHC (P)

          Immunohistochemical analysis of Podoplanin was performed using formalin-fixed paraffin-embedded mouse lung and mouse skeletal muscle tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degre... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Podoplanin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Podoplanin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Podoplanin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Podoplanin Antibody in Western Blot (WB)
          Podoplanin Antibody in Western Blot (WB)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed), Flow Cytometry (ICC/IF, IHC (PFA), Flow)
          Podoplanin Antibody in Immunocytochemistry (ICC/IF)
          Podoplanin Antibody in Immunocytochemistry (ICC/IF)
          Podoplanin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Podoplanin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Podoplanin Antibody in Immunocytochemistry (ICC/IF)
          Podoplanin Antibody in Immunocytochemistry (ICC/IF)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Immunohistochemistry (IHC)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody in Flow Cytometry (Flow)
          Podoplanin Antibody
          Podoplanin Antibody
          Podoplanin Antibody

          Product Details

          14-5381-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          Assay-Dependent
          View 22 publications 22 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 11 publications 11 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 20 publications 20 publications

          Immunoprecipitation (IP)

          Assay-Dependent
          -

          Functional Assay (Functional)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Published species

          Hamster, Human, Mouse, Rat

          Host/Isotype

          Syrian hamster / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          eBio8.1.1 (8.1.1)

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Biotin 
          • eFluor 660
          • PE 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Super Bright 600
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1210505

          Product Specific Information

          Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.

          Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

          Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of TE-71 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: aggrus; CTA-520D8.1; E11; E11 antigen epitope; epithelial cell surface transmembrane protein antigen; Glycoprotein 38; Gp38; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; Podoplanin; pulmonary type I alveolar epithelial cell transmembrane differentiation marker; RP23-348F1.2; RTI140; T1-alpha; T1A; Transmembrane glycoprotein E11; Type I cell 40 kDa protein

          View more View less

          Gene Aliases: E11; Gp38; OTS-8; Ots8; Pdpn; RANDAM-2; RTI40; T1-alpha; T1a; T1alpha

          View more View less

          UniProt ID: (Rat) Q64294, (Mouse) Q62011

          View more View less

          Entrez Gene ID: (Rat) 54320, (Mouse) 14726

          View more View less

          Function(s)
          molecular_function water transmembrane transporter activity folic acid transporter activity amino acid transmembrane transporter activity water channel activity
          Process(es)
          cell morphogenesis lymphangiogenesis prostaglandin metabolic process water transport amino acid transport inflammatory response cell adhesion signal transduction nervous system development cell proliferation regulation of cell shape positive regulation of platelet activation folic acid transport single organismal cell-cell adhesion lung development positive regulation of cell migration tube morphogenesis lung alveolus development positive regulation of cellular component movement response to hyperoxia regulation of G1/S transition of mitotic cell cycle movement of cell or subcellular component multicellular organism development visceral serous pericardium development
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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