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Invitrogen
Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 13
Podoplanin Antibody (46-5381-82) in Flow
TE-71 cells were stained with 0.125 µg of Syrian Hamster IgG Isotype Control, PerCP-eFluor 710 (Product # 46-4914-80) (blue histogram) or 0.125 µg of Podoplanin Monoclonal Antibody, PerCP-eFluor 710 (purple histogram). Viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
46-5381-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Syrian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio8.1.1 (8.1.1)
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848339
Product Specific Information
Description: The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression in kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.
Applications Reported: This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of the TE-71 cell line. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Target Information
Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aggrus; CTA-520D8.1; E11; Glycoprotein 38; Gp38; OTS-8; OTTHUMP00000009640; OTTHUMP00000044504; PA2.26 antigen; Podoplanin; RP23-348F1.2; Transmembrane glycoprotein E11
Gene Aliases: Gp38; OTS-8; Ots8; Pdpn; RANDAM-2; T1-alpha; T1a; T1alpha
UniProt ID: (Mouse) Q62011
Entrez Gene ID: (Mouse) 14726
cell morphogenesis
lymphangiogenesis
prostaglandin metabolic process
water transport
amino acid transport
movement of cell or subcellular component
cell adhesion
signal transduction
multicellular organism development
cell proliferation
regulation of cell shape
positive regulation of platelet activation
folic acid transport
single organismal cell-cell adhesion
lung development
positive regulation of cell migration
tube morphogenesis
lung alveolus development
positive regulation of cellular component movement
visceral serous pericardium development
regulation of G1/S transition of mitotic cell cycle
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