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Pogz Antibody (600-401-A89) in WB
Western blot using Rocklands affinity purified anti-Pogz antibody shows detection of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:20,000. Personal Communication, K.O. Gudmundsson and J. Keller, NCI, Frederick, MD.
Product Details
600-401-A89
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Baboon,
Bovine,
Dog,
Chicken,
Chimpanzee,
Human,
Marsupial,
Mouse,
Rhesus monkey,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of mouse Pogz protein.
Conjugate
Form
Liquid
Concentration
1.6 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is known to react with mouse Pogz protein. A BLAST analysis was used to suggest cross-reactivity with Pogz from human, dog, short-tailed opossum, cattle, rat, chimpanzee, macaque, olive baboon, and chicken sources based on 100% homology with the immunizing sequence. Reactivity with Pogz from other sources has not been determined.
Target Information
POGZ appears to be a zinc finger protein containing a transposase domain at the C-terminus. This protein was found to interact with the transcription factor SP1 in a yeast two-hybrid systemThe protein encoded by this gene appears to be a zinc finger protein containing a transposase domain at the C-terminus. This protein was found to interact with the transcription factor SP1 in a yeast two-hybrid system. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Pogo transposable element with ZNF domain; putative protein product of Nbla00003; Suppressor of hairy wing homolog 5; Zinc finger protein 280E; Zinc finger protein 635
Gene Aliases: 9530006B08Rik; BOS_2943; KIAA0461; MRD37; Nbla00003; POGZ; SUHW5; WHSUS; ZNF280E; ZNF635; ZNF635m
UniProt ID: (Human) Q7Z3K3, (Mouse) Q8BZH4
Entrez Gene ID: (Human) 23126, (Rhesus monkey) 710634, (Bovine) 535254, (Dog) 100856450, (Mouse) 229584, (Rat) 310658
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