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Invitrogen
Pokemon (LRF) Monoclonal Antibody (13E9), eFluor™ 660, eBioscience™
FIGURE: 1 / 2
Pokemon (LRF) Antibody (50-3309-80) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL Armenian Hamster IgG Isotype Control eFluor® 660 (left) or 5 µg/mL Anti-Human Pokeman (LRF) eFluor® 660 (right). Nuclei are stained with DAPI (colocalization appears pink).
Product Details
50-3309-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
13E9
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574200
Product Specific Information
Description: This monoclonal antibody reacts with human Leukemia/lymphoma-related factor (LRF). This 65-kDa protein is also known as POKEMON, FBI-1, and Zbtb7a. LRF is a member of the POK family of transcriptional repressors, and is expressed in hematopoietic stem cells, T and B lymphocytes, macrophages, and erythroid cells. This protein is also highly expressed in human non-Hodgkin's lymphoma tissues and diffuse large B-cell lymphomas, demonstrating its prominent role in oncogenic transformation. Studies have demonstrated that LRF is also involved in B cell lineage commitment and development by opposing Notch signaling. Moreover, LRF is critical for erythroid terminal differentiation in the bone marrow by inhibiting Bim-mediated apoptosis. Finally, LRF interacts with Bcl-6 in germinal center B cells.
Applications Reported: This 13E9 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This 13E9 antibody has been tested by immunohistochemistry on human FFPE tissue using high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp547O146; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; HIV-1 1st-binding protein 1; HIV-1 inducer of short transcripts binding protein; Leukemia/lymphoma-related factor; lymphoma related factor; MGC99631; POK erythroid myeloid ontogenic factor; POZ and Krueppel erythroid myeloid ontogenic factor; TIP21; TTF-I-interacting peptide 21; zi; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein; Zinc finger and BTB domain-containing protein 7A; Zinc finger protein 857A
Gene Aliases: FBI-1; FBI1; LRF; pokemon; TIP21; ZBTB7; ZBTB7A; ZNF857A
UniProt ID: (Human) O95365
Entrez Gene ID: (Human) 51341
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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