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Invitrogen
PrP Monoclonal Antibody (F89/160.1.5)
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PrP Antibody (MA1-750) in ICC/IF
Immunofluorescence analysis of PrP was performed using 70% confluent log phase A549 or MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Anti-PrP Monoclonal Antibody (F89/160.1.5) (Product # MA1-750) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
MA1-750
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Deer,
Human,
Mouse,
Sheep
Published species
Hamster,
Human,
Sheep
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
F89/160.1.5
Immunogen
Synthetic peptide corresponding to residues S(146) R P L I H F G S D Y E D R(159) of bovine PrP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_325438
Product Specific Information
MA1-750 detects prion protein (PrP) protein from a variety of species that have the conserved n-IHFG-c epitope, including agriculturally important animal species such as sheep, bovine, deer, and elk. MA1-750 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects a 33-35 kDa protein from normal animals and a 27-30 kDa protein which represents PrP in brain protease treated tissue extracts from infected animals. Immunohistochemical staining of PrP(Sc) in infected sheep brain with MA1-750 results in intense staining of the spongiform lesions. In immunohistochemical procedures this antibody detects only the PrP(Sc) and not PrP(C). The MA1-750 immunogen is a synthetic peptide corresponding to residues S(146) R P L I H F G S D Y E D R(159) of bovine PrP. This peptide (Cat. # PEP-051) is available for use in neutralization and control experiments. MA1-750 is known to specifically recognize a conserved epitope of the PrP(Sc) protein comprising the amino acids n-IHFG-c.
Target Information
Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Alternative prion protein; AltPrP; ASCR; BSE; CD230; CD230 antigen; Major prion protein; Major scrapie-associated fibril protein 1; MGC26679; PK resistant core; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia); prion protein precursor PrP; prion protein PrP; prion protein variant a; prion protein variant b; prion-related protein; PrP; PrP27-30; PrP33-35C; PrPc; TSE
Gene Aliases:
AA960666; AI325101; ALTPRP; ASCR; BOS_13027; CD230; CJD; GSS; KURU; p27-30; PRIP; Prn-i; Prn-p; PRNP; PRP; PrP27-30; PrP33-35C; PrP
UniProt ID: (Bovine) P10279, (Human) P04156, (Sheep) P23907, (Mouse) P04925
Entrez Gene ID: (Bovine) 281427, (Human) 5621, (Sheep) 493887, (Mouse) 19122
negative regulation of protein phosphorylation
response to oxidative stress
negative regulation of interferon-gamma production
negative regulation of interleukin-17 production
negative regulation of interleukin-2 production
negative regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of activated T cell proliferation
negative regulation of T cell receptor signaling pathway
protein homooligomerization
negative regulation of calcineurin-NFAT signaling cascade
nucleobase-containing compound metabolic process
cellular copper ion homeostasis
learning or memory
regulation of protein localization
cellular response to drug
response to cadmium ion
response to copper ion
cellular response to copper ion
regulation of potassium ion transmembrane transport
cell cycle arrest
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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