Invitrogen

Pre-Pro-Vasopressin Polyclonal Antibody

Promotion

View available promotion(s)

Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-143640

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000
-

Immunocytochemistry (ICC/IF)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to key amino acid residues surrounding the cleavage sites for the human vasopressin precursor peptide, conjugated to keyhole limpet hemocyanin (KLH).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

10mM HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 150mM NaCl

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942868

Product Specific Information

Specific for locally produced levels of the ~ 20 kDa uncleaved pre-pro-vasopressin protein. Immunolabeling of the uncleaved pre-pro-vasopressin protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.

Target Information

Vasopressin is a nine amino-acid peptide hormone that plays a key role in water and blood pressure homeostasis (Qureshi S., et al 2014). Vasopressin is the end-product of a highly processed 164 amino acid pre-pro-peptide. Processing of the vasopressin pre-pro-peptide results in three distinct peptides with a 1:1:1 ratio: vasopressin, neurophysin II, and copeptin (Arroyo J.P. et al, 2021). Vasopressin is the biologically active hormone, neurophysin II is a carrier molecule for vasopressin, and copeptin is the c-terminal glycosylated end-product. Vasopressin has been thought to be primarily made in the brain, and the sole source of vasopressin stimulating vasopressin V2 receptors in the kidney until recent studies (Arroyo JP, et al 2022). This antibody specially detects uncleaved pre-pro-vasopressin, accurately identifying locally produced vasopressin versus peripheral uptake of hypothalamic vasopressin or related peptides (Arroyo J.P. et al, 2022). This key research tool aided the Arroyo group in discovering immunoreactive vasopressin outside of the brain by recognizing localized vasopressin in human and mouse kidney, specifically in the distal nephron.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: antidiuretic hormone; arginine vasopressin (Diabetes insipidus), same as Di (conflicting physical mapping); ARVP; AVP-NPII; copeptin; neurohypophyseal; prepro-arginine-vasopressin-neurophysin II; prepro-AVP-NP II; prepropressophysin; preprovasopressin-neurophysin; Vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin II-copeptin; vasopressin-neurophysin prepropeptide; VP

View more View less

Gene Aliases: ADH; ARVP; AVP; AVP-NPII; AVRP; DI; Vas; VP; Vsp

View more View less

UniProt ID: (Human) P01185, (Mouse) P35455, (Rat) P01186

View more View less

Entrez Gene ID: (Human) 551, (Mouse) 11998, (Rat) 24221

View more View less

Function(s)
protein kinase activity signal transducer activity receptor binding neuropeptide hormone activity neurohypophyseal hormone activity V1A vasopressin receptor binding V1B vasopressin receptor binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process hormone activity protein binding V2 vasopressin receptor binding
Process(es)
maternal aggressive behavior positive regulation of systemic arterial blood pressure signal transduction positive regulation of cytosolic calcium ion concentration negative regulation of female receptivity grooming behavior locomotory behavior positive regulation of cell proliferation positive regulation of gene expression positive regulation of glutamate secretion positive regulation of cell growth positive regulation of cAMP biosynthetic process positive regulation of prostaglandin biosynthetic process positive regulation of cellular pH reduction positive regulation of peptidyl-serine phosphorylation social behavior regulation of renal sodium excretion vasoconstriction maternal behavior negative regulation of apoptotic process penile erection negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of vasoconstriction regulation of blood vessel size multicellular organismal water homeostasis negative regulation of transmission of nerve impulse ERK1 and ERK2 cascade protein kinase C signaling negative regulation of release of cytochrome c from mitochondria renal water homeostasis generation of precursor metabolites and energy water transport apoptotic process cell-cell signaling response to testosterone response to nicotine hyperosmotic salinity response sodium-independent organic anion transport response to ethanol temperature homeostasis protein phosphorylation smooth muscle contraction response to organic cyclic compound
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support