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Invitrogen
Profilin 1 Polyclonal Antibody
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FIGURE: 1 / 8
Profilin 1 Antibody (PA5-27612) in ICC/IF
Immunofluorescence analysis of Profilin 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Profilin 1 Polyclonal Antibody (Product # PA5-27612) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ce... View More
Product Details
PA5-27612
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Profilin 1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.56 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545088
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, mouse spleen, Rat spleen.
Predicted reactivity: Mouse (95%), Rat (95%), Pig (94%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: actin binding protein; Epididymis tissue protein Li 184a; Profilin I; Profilin-1
Gene Aliases: ALS18; Pfn; PFN1
UniProt ID: (Human) P07737, (Rat) P62963, (Mouse) P62962
Entrez Gene ID: (Human) 5216, (Rat) 64303, (Mouse) 18643
neural tube closure
regulation of transcription from RNA polymerase II promoter
actin polymerization or depolymerization
response to organic substance
positive regulation of epithelial cell migration
negative regulation of actin filament polymerization
positive regulation of actin filament polymerization
negative regulation of actin filament bundle assembly
positive regulation of ATPase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
positive regulation of DNA metabolic process
positive regulation of stress fiber assembly
negative regulation of stress fiber assembly
cellular response to growth factor stimulus
positive regulation of ruffle assembly
actin cytoskeleton organization
positive regulation of actin filament bundle assembly
protein stabilization
Wnt signaling pathway, planar cell polarity pathway
cell-cell adhesion
regulation of actin polymerization or depolymerization
sequestering of actin monomers
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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