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Invitrogen
Progerin Monoclonal Antibody (13A4D4)
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Progerin Antibody (MA5-18073) in WB
Western blot analysis of Progerin in Hela cells expressing flag-tagged progerin using a Progerin monoclonal antibody (Product # MA5-18073) at various dilutions (lanes 1-3) compared to a control Lamin A/C antibody (lane 4) that recognizes Lamin A and C as well as eptopic progerin. Hela samples were boiled in Laemmli buffer, separated by SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was blocked in 3% NFDM-PBST for 1 hr at room temp, incubated at 4ºC in 0.5% NFDM-PBST, and detected using an HRP-conjugated Fc-specific ant... View More
Product Details
MA5-18073
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
13A4D4
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 604-611 (GAQSPQNC) at the C terminus of Human Progerin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539456
Product Specific Information
This antibody specifically recognizes Progerin (Lamin A delta50) and does not cross-react with endogenous prelamin A.
Western blot results show a band at approximately 70 kDa.
Target Information
Progerin is a 614 amino acid protein involved in Hutchinson-Gilford progeria syndrome. Progerin is most often generated by a point mutation (C1824T) in the LMNA gene that codes for lamin-A/C. This mutation activates a cryptic splice site and gives rise to a form of lamin A with a 50-amino acid internal deletion within the C-terminal domain of the protein. Approximately 80% of Hutchinson-Gilford progeria syndrome cases carry a single copy of the most common mutation, a silent point mutation, G608G (GGC > GGT), within exon 11 of the LMNA gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70 kDa lamin; lamin A/C-like 1; Lamin AC; Mutant lamin A/C; Prelamin-A/C; progerin mutant; renal carcinoma antigen NY-REN-32
Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1
UniProt ID: (Human) P02545
Entrez Gene ID: (Human) 4000
mitotic nuclear envelope disassembly
mitotic nuclear envelope reassembly
spermatogenesis
response to mechanical stimulus
regulation of cell migration
establishment or maintenance of microtubule cytoskeleton polarity
protein localization to nucleus
sterol regulatory element binding protein import into nucleus
IRE1-mediated unfolded protein response
positive regulation of osteoblast differentiation
ventricular cardiac muscle cell development
cellular response to hypoxia
negative regulation of release of cytochrome c from mitochondria
positive regulation of cell aging
regulation of protein localization to nucleus
negative regulation of adipose tissue development
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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