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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Progesterone Receptor Antibodies

          Invitrogen

          Progesterone Receptor Monoclonal Antibody (SP2)

          Advanced Verification
          4 Published Figures
          134 References
          View all (148) Progesterone Receptor antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Progesterone Receptor Monoclonal Antibody (SP2)

          • Antibody Testing Data (7)
          • Published Figures (4)
          • Advanced Verification (3)
          Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          Progesterone Receptor Antibody (MA5-14505) in ICC/IF

          Immunofluorescence analysis of Progesterone Receptor was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Progesterone Receptor (SP2) Rabbit Polyclonal Antibody (Product # MA5-14505) at 2 µg/mL in 0.1% BSA and incu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)
          Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)
          Progesterone Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Progesterone Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Progesterone Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Progesterone Receptor Antibody in Western Blot (WB)
          Progesterone Receptor Antibody in Western Blot (WB)
          Progesterone Receptor Antibody in Immunohistochemistry (IHC)
          Progesterone Receptor Antibody in Immunohistochemistry (IHC)
          Progesterone Receptor Antibody in Immunohistochemistry (IHC)
          Progesterone Receptor Antibody in Immunohistochemistry (IHC)
          Progesterone Receptor Antibody
          Progesterone Receptor Antibody
          Progesterone Receptor Antibody

          Product Details

          MA5-14505

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:2,000
          View 14 publications 14 publications

          Immunohistochemistry (IHC)

          -
          View 104 publications 104 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:400
          View 9 publications 9 publications

          Immunocytochemistry (ICC/IF)

          1:100
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          1:100
          -

          Miscellaneous PubMed (MISC)

          -
          View 5 publications 5 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Dog, Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Monoclonal

          Type

          Antibody

          Clone

          SP2

          Immunogen

          Recombinant protein encoding human progesterone receptor 412-526 aa
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.08 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.2, with 1% BSA

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10980030

          Product Specific Information

          MA5-14505 targets Progesterone Receptor in IHC (P) and WB applications and shows reactivity with Human samples.

          MA5-14505 has been successfully used in western blotting, immunofluorescence, and immunohistochemistry analysis of Progesterone Receptor in human and mouse samples.

          The MA5-14505 immunogen is recombinant protein encoding human progesterone receptor 412-526 aa.

          Target Information

          The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor

          View more View less

          Gene Aliases: NR3C3; PGR; PR

          View more View less

          UniProt ID: (Human) P06401

          View more View less

          Entrez Gene ID: (Human) 5241

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid hormone receptor activity steroid binding protein binding zinc ion binding enzyme binding ATPase binding
          Process(es)
          transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling negative regulation of gene expression steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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