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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Progesterone Receptor Antibodies

          Invitrogen

          Progesterone Receptor Monoclonal Antibody (hPRa 2)

          Advanced Verification
          57 References
          View all (148) Progesterone Receptor antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite Progesterone Receptor Monoclonal Antibody (hPRa 2)

          • Antibody Testing Data (6)
          • Advanced Verification (1)
          Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)
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          Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          Progesterone Receptor Antibody (MA5-12642) in ICC/IF

          Immunofluorescent analysis of Progesterone Receptor (green) in HeLa cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with Progesterone Receptor mouse mono... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Progesterone Receptor Antibody in Immunocytochemistry (ICC/IF)
          Progesterone Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Progesterone Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Progesterone Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Progesterone Receptor Antibody in Western Blot (WB)
          Progesterone Receptor Antibody in Western Blot (WB)
          Progesterone Receptor Antibody

          Product Details

          MA5-12642

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:50-1:100
          View 11 publications 11 publications

          Immunohistochemistry (IHC)

          -
          View 38 publications 38 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:100
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Bovine, Cat, Chicken, Deer, Horse, Human, Mouse, Rat, Rhesus monkey

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          hPRa 2

          Immunogen

          PgR from a human endometrial carcinoma (EnCa 101) grown in athymic mice
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.4 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10981830

          Product Specific Information

          MA5-12642 targets Progesterone Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with Bovine, Chicken, Equine, Human, and Rabbit samples.

          The MA5-12642 immunogen is pgR from a human endometrial carcinoma (EnCa 101) grown in athymic mice.

          Target Information

          The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor

          View more View less

          Gene Aliases: NR3C3; PGR; PR

          View more View less

          UniProt ID: (Human) P06401

          View more View less

          Entrez Gene ID: (Human) 5241

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid hormone receptor activity steroid binding protein binding zinc ion binding enzyme binding ATPase binding
          Process(es)
          transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling negative regulation of gene expression steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter
          It has to be done as per old AB suggested Products section.
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