Invitrogen

Prohibitin Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Product Details

PA5-95085

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Prohibitin (240-256aa KLEAAEDIAYQLSRSRN).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide, 0.05mg thimerosal

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806891

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: Rat Lung Tissue, Rat Skeletal Muscle Tissue, Rat Brain Tissue, Rat Kidney Tissue, HELA Cell, MCF-7 Cell, PC-12 Cell, A549 Cell, SMMC Cell, mouse NIH3T3 whole cell. IHC: Human Lung Cancer Tissue, rat brain tissue. ICC/IF: U20S cell, HELA Cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-cell receptor-associated protein 32; BAP 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; prohibitin; Prohibitin 1

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Gene Aliases: Bap32; HEL-215; HEL-S-54e; PHB; PHB1

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UniProt ID: (Human) P35232, (Mouse) P67778, (Rat) P67779

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Entrez Gene ID: (Human) 5245, (Mouse) 18673, (Rat) 25344

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding complement component C3a binding complement component C3b binding protein binding protein C-terminus binding enzyme binding proteinase activated receptor binding histone deacetylase binding transcription regulatory region DNA binding molecular_function
Process(es)
negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation regulation of transcription, DNA-templated mitochondrion organization signal transduction negative regulation of cell proliferation negative regulation of transcription by competitive promoter binding histone deacetylation negative regulation of cell growth negative regulation of protein catabolic process regulation of apoptotic process positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of complement activation protein stabilization progesterone receptor signaling pathway negative regulation of androgen receptor signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to interleukin-6 DNA biosynthetic process negative regulation of glucocorticoid receptor signaling pathway transcription from RNA polymerase II promoter negative regulation of apoptotic process ovarian follicle development ovarian follicle atresia organ regeneration response to cytokine response to immobilization stress response to drug response to peptide hormone response to ethanol
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