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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Prohibitin Antibodies

          Invitrogen

          Prohibitin Monoclonal Antibody (II-14-10)

          Advanced Verification
          7 Published Figures
          59 References
          View all (53) Prohibitin antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite Prohibitin Monoclonal Antibody (II-14-10)

          • Antibody Testing Data (4)
          • Published Figures (7)
          • Advanced Verification (1)
          Prohibitin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Prohibitin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          Prohibitin Antibody (MA5-12858) in IHC (P)

          Formalin-fixed, paraffin-embedded human heart stained with Prohibitin antibody using peroxidase-conjugate and DAB chromogen. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Prohibitin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody in Western Blot (WB)
          Prohibitin Antibody

          Product Details

          MA5-12858

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5-1.0 µg/mL
          View 44 publications 44 publications

          Immunohistochemistry (IHC)

          -
          View 6 publications 6 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          2-4 µg/mL
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          -
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chicken, Human, Mouse, Non-human primate, Pig, Rat

          Published species

          Avian, Human, Mouse, Pig, Rat, Virus

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          II-14-10

          Immunogen

          Purified recombinant rat prohibitin protein

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10980219

          Product Specific Information

          MA5-12858 targets Prohibitin in immunofluorescence, immunohistochemistry (paraffin), and Western blot applications and shows reactivity with Chicken, Human, mouse, Porcine, and Rat samples.

          The MA5-12858 immunogen is purified recombinant rat prohibitin protein.

          Target Information

          Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: B-cell receptor-associated protein 32; BAP 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; prohibitin; Prohibitin 1

          View more View less

          Gene Aliases: Bap32; HEL-215; HEL-S-54e; PHB; PHB1

          View more View less

          UniProt ID: (Human) P35232, (Mouse) P67778, (Rat) P67779

          View more View less

          Entrez Gene ID: (Human) 5245, (Chicken) 419980, (Mouse) 18673, (Rat) 25344

          View more View less

          Function(s)
          RNA polymerase II transcription factor activity, sequence-specific DNA binding complement component C3a binding complement component C3b binding protein binding protein C-terminus binding enzyme binding proteinase activated receptor binding histone deacetylase binding transcription regulatory region DNA binding molecular_function
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter osteoblast differentiation regulation of transcription, DNA-templated mitochondrion organization signal transduction negative regulation of cell proliferation negative regulation of transcription by competitive promoter binding histone deacetylation negative regulation of cell growth negative regulation of protein catabolic process regulation of apoptotic process positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of complement activation protein stabilization progesterone receptor signaling pathway negative regulation of androgen receptor signaling pathway negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to interleukin-6 DNA biosynthetic process negative regulation of glucocorticoid receptor signaling pathway transcription from RNA polymerase II promoter negative regulation of apoptotic process DNA replication ovarian follicle development ovarian follicle atresia organ regeneration response to cytokine response to immobilization stress response to drug response to peptide hormone response to ethanol
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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