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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Prolactin Receptor Antibodies

          Invitrogen

          Prolactin Receptor Monoclonal Antibody (1A2B1)

          Advanced Verification
          35 Published Figures
          46 References
          View all (35) Prolactin Receptor antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Prolactin Receptor Monoclonal Antibody (1A2B1)

          • Antibody Testing Data (2)
          • Published Figures (35)
          • Advanced Verification (1)
          Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 38

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          Prolactin Receptor Antibody (35-9200) in ICC/IF

          Immunofluorescence analysis of Prolactin Receptor was performed using 70% confluent log phase SW40 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Prolactin Receptor (ECD) (1A2B1) Monoclonal Antibody (Product # 35-9200) at 2µg/mL in 0.1% BSA and incub... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
          Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
          Prolactin Receptor Antibody in Immunocytochemistry (ICC/IF)
          Prolactin Receptor Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Prolactin Receptor Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Prolactin Receptor Antibody in Immunohistochemistry (IHC)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot, Flow Cytometry (WB, Flow)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot, Immunoprecipitation (WB, IP)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot, Immunoprecipitation (WB, IP)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Western Blot (WB)
          Prolactin Receptor Antibody in Proximity Ligation Assay (PLA) (PLA)
          Prolactin Receptor Antibody

          Product Details

          35-9200

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-3 µg/mL
          View 21 publications 21 publications

          Immunohistochemistry (IHC)

          5-10 µg/mL
          View 8 publications 8 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          View 5 publications 5 publications

          Flow Cytometry (Flow)

          -
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          5-10 µg/mL
          View 3 publications 3 publications

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          in situ PLA (PLA)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Hamster, Human, Mouse

          Host/Isotype

          Mouse / IgG2b, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1A2B1

          Immunogen

          Recombinant human prolactin receptor protein containing the extracellular domain (ECD).

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533231

          Product Specific Information

          This antibody is predicted to react with mouse and rat and reacts with the long form of the human prolactin receptor (hPRLr) protein at ~85-90 kDa, and may also identify the human prolactin receptor intermediate and delta S1 isoforms by western blotting. On immunoprecipitation, this antibody strongly recognizes only the long form of hPRLr. During development reactivity was confirmed with human T-47D breast carcinoma cells.

          Target Information

          Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: hPRL receptor; Prl R; PRL-R; Prolactin receptor; secreted prolactin binding protein

          View more View less

          Gene Aliases: HPRL; hPRLrI; MFAB; PRLR

          View more View less

          UniProt ID: (Human) P16471

          View more View less

          Entrez Gene ID: (Human) 5618

          View more View less

          Function(s)
          prolactin receptor activity protein binding peptide hormone binding protein homodimerization activity ornithine decarboxylase activator activity metal ion binding
          Process(es)
          steroid biosynthetic process cell surface receptor signaling pathway activation of transmembrane receptor protein tyrosine kinase activity embryo implantation lactation regulation of cell adhesion regulation of epithelial cell differentiation prolactin signaling pathway T cell activation activation of JAK2 kinase activity negative regulation of apoptotic process JAK-STAT cascade involved in growth hormone signaling pathway mammary gland epithelial cell differentiation prostate gland growth mammary gland alveolus development
          It has to be done as per old AB suggested Products section.
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